HPD Mediated Photochemotherapy for Selective Treatment of Leukemic Cells VS Normal Cells
As photochemotherapy of solid tumors is rapidly develloping recent studies suggested that in-vitro photochemotherapy mediated by HPD could be more efficient on leukemic cells than normal cells (1), (2) and this represent a way of cell selection. In order to demonstrate this phenomenon we studied the phototoxicity on both leukemic cells (L1210) and normal syngeneic hemoprogenitors. We varied three culture parameters: time incubation of the cells with HPD, temperature of the medium during incubation and treatment of the cells, laser dose fractionation in 2 or 3. For each condition 3 separates experiments in triplicate have been performed, and each experiment involved simultaneously both cell types for 2 or 3 laser energy levels.
KeywordsFractionation Neuroblastoma Cyanine
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