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HPD Mediated Photochemotherapy for Selective Treatment of Leukemic Cells VS Normal Cells

  • T. Patrice
  • M. T. Foultier
  • V. Praloran
  • D. Cloarec
  • L. Le Bodic
Conference paper

Abstract

As photochemotherapy of solid tumors is rapidly develloping recent studies suggested that in-vitro photochemotherapy mediated by HPD could be more efficient on leukemic cells than normal cells (1), (2) and this represent a way of cell selection. In order to demonstrate this phenomenon we studied the phototoxicity on both leukemic cells (L1210) and normal syngeneic hemoprogenitors. We varied three culture parameters: time incubation of the cells with HPD, temperature of the medium during incubation and treatment of the cells, laser dose fractionation in 2 or 3. For each condition 3 separates experiments in triplicate have been performed, and each experiment involved simultaneously both cell types for 2 or 3 laser energy levels.

Keywords

Leukemic Cell Laser Treatment Dose Fractionation Laser Exposure Selective Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Effects of laser irradiation on hernatoporphyrin treated normal and transformed thyroid cells in culture. A. Andreoni, R. Cubeddu, S. Desilvestri et Al Cancer Res.: 43, 2076–2080. 1983Google Scholar
  2. Uptake of hernatoporphyrin derivative by normal and malignant cells: Effects of serum, ph, temperature, and cell size R. Böhmer, G. Morsty Cancer Res.: 45, 5328–5334, 1985Google Scholar
  3. Photodynamic inactivation of cancer cells in-vitro: Effects of irradiation, temperature and dose fractionation J. Moan, T. Christensen Cancer Lett.: 6, 331–335, 1979Google Scholar
  4. Dye-mediated photosensitization of murine neuroblastoma cells F. Sieber, M. Sieber-Blum Cancer Res.: 46, 2072–2076, 1986Google Scholar

Copyright information

© Springer-Verlag Berlin, Heidelberg 1988

Authors and Affiliations

  • T. Patrice
    • 1
  • M. T. Foultier
    • 1
  • V. Praloran
    • 1
  • D. Cloarec
    • 1
  • L. Le Bodic
    • 1
  1. 1.Departement LaserHopital G et R. LaennecNantes CedexFrance

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