Histological Analysis of Cottontail Rabbit Papilloma Virus-Induced Papillomas Treated with Hematoporphyrin Photodynamic Therapy

  • Mark J. Shikowitz
  • Bettie M. Steinberg
  • Rachel L. Galli
  • Allan L. Abramson
Conference paper

Abstract

Our findings of papilloma regression following treatment with HPD-PDT have been reported recently (Shikowitz et.al., 1986). HPD has been shown to localize selectively in rapidly growing tissue such as malignancies and papillomas. Henderson et.al. (1980) demonstrated the ability of HPD to localize in both squamous cell carcinomas and chemically induced papillomas raised on the shaved backs of Swiss white mice by painting with 9,10-dimethyl-1,2-benzanthracene and croton oil. This prompted our initial investigation into the possible efficacy of HPD-PDT for the treatment of virally induced papilloma disease, utilizing CRPV as a model system. We successfully treated large cutaneous rabbit papillomas with intravenous administration of HPD followed by activation with light at 630 nm from an Argon pump dye laser. Two animals were maintained for 18 months following treatment. The animals were sacrificed and biopsies were taken from totally regressed papillomas, partially regressed papillomas, untreated papillomas and normal skin. The histological analysis with molecular analysis confirmation form the basis of this report.

Keywords

Formalin Cage Argon Hematoxylin Eosin 

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Copyright information

© Springer-Verlag Berlin, Heidelberg 1988

Authors and Affiliations

  • Mark J. Shikowitz
    • 1
  • Bettie M. Steinberg
    • 1
  • Rachel L. Galli
    • 1
  • Allan L. Abramson
    • 1
  1. 1.Department of Otolaryngology and Communicative DisordersLong Island Jewish Medical CenterNew Hyde ParkUSA

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