Nephron- and Collecting Duct Structure in the Kidney, Rat

  • S. Bachmann
  • T. Sakai
  • W. Kriz


The rat kidney is a unipapillary kidney. In histologic sections the different regions of this kidney are well discernible, since the tubules as well as the blood vessels are arranged in a regular zonal pattern. The renal cortex, as a whole, is cup-shaped, with inverted margins, and surrounds the renal medulla.


Mesangial Cell Distal Convoluted Tubule Outer Medulla Slit Diaphragm Intercalate Cell 
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  1. Al-Awqati Q, Schwartz GJ (1985) Plasticity in epithelial polarity. In: Frömter E (ed) 5th European colloquium on renal physiology. Frankfurt, p 7 (abstract)Google Scholar
  2. Allen F, Tisher CC (1976) Morphology of the ascending thick limb of Henle. Kidney Int 9: 8–22PubMedCrossRefGoogle Scholar
  3. Andrews PM, Bates SB (1984) Filamentous actin bundles in the kidney. Anat Rec 210: 1–9PubMedCrossRefGoogle Scholar
  4. Ausiello DA, Kreisberg JI, Roy C, Karnovsky MJ (1980) Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin. J Clin Invest 65:754–760PubMedCrossRefGoogle Scholar
  5. Bachmann S, Kriz W (1982) Histotopography and ultrastructure of the thin limbs of the loop of Henle in the hamster. Cell Tissue Res 225: 111–127PubMedCrossRefGoogle Scholar
  6. Bachmann S, Koeppen-Hagemann I, Kriz W (1985) Ultrastructural localization of Tamm-Horsfall glycoprotein (THP) in rat kidney as revealed by protein A-gold immunocytochemistry. In: Frömter E (ed) 5th European colloquium on renal physiology. Frankfurt, p 158 (abstract)Google Scholar
  7. Baeyer H von, Deetjen P (1985) Renal glucose transport. In: Seldin DW, Giebisch G (eds) The kidney: physiology and pathophysiology. Raven, New York, pp 1663–1675Google Scholar
  8. Baines AD, de Rouffignac C (1969) Functional heterogeneity of nephrons. II. Filtration rates, intraluminal flow velocities and fractional water reabsorption. Pflugers Arch 308: 260–276PubMedCrossRefGoogle Scholar
  9. Barajas L (1966) The development and ultrastructure of the juxtaglomerular cell granules. J Ultrastruct Res 15: 400–413PubMedCrossRefGoogle Scholar
  10. Barajas L (1970) The ultrastructure of the juxtaglomerular apparatus as disclosed by three-dimensional reconstructions from serial sections: the anatomical relationship between the tubular and vascular components. J Ultrastruct Res 33: 116–147PubMedCrossRefGoogle Scholar
  11. Barajas L (1971) Renin secretion: an anatomical basis for tubular control. Science 172: 484–487CrossRefGoogle Scholar
  12. Barajas L (1981) The juxtaglomerular apparatus: anatomical considerations in feedback control of glomerular filtration rate. Fed Proc 40: 78–86PubMedGoogle Scholar
  13. Barajas L, Latta H (1963) A three-dimensional study of the juxtaglomerular apparatus in the rat. Light and electron microscopic observations. Lab Invest 12: 257–269PubMedGoogle Scholar
  14. Becker B (1978) Quantitative Beschreibung der Innenzone der Rattenniere. Inaugural dissertation, Münster UniversityGoogle Scholar
  15. Boulpaep EL (1978) Electrophysiology of the kidney. In: Giebisch G (ed) Transport organs. Springer, Berlin Heidelberg New York, pp 97–144 (Membrane transport in biology, vol 4 )Google Scholar
  16. Bucher O, Kaissling B (1973) Morphologie des Juxtaglomeruiaren Apparates. Verh Anat Ges 67: 109–136Google Scholar
  17. Christensen JA, Bohle A (1978) The juxtaglomerular apparatus in the normal rat kidney. Virchows Arch [A] 379: 143–150CrossRefGoogle Scholar
  18. Christensen JA, Bjoerke HA, Meyer DS, Bohle A (1979) The normal juxtaglomerular apparatus in the human kidney. A morphological study. Acta Anat (Basel) 103: 374–383CrossRefGoogle Scholar
  19. Crayen ML, Thoenes W (1978) Architecture and cell structures in the distal nephron of the rat kidney. Cytobiology 17: 197–211Google Scholar
  20. Ernst SA (1975) Transport ATPase cytochemistry: ultrastructural localization of potassium-dependent and potassium-independent activities in rat kidney cortex. J Cell Biol 66: 586–608PubMedCrossRefGoogle Scholar
  21. Ernst SA, Schreiber JH (1981) Ultrastructural localization of Na+, K+-ATPase in rat and rabbit kidney medulla. J Cell Biol 91: 803–813PubMedCrossRefGoogle Scholar
  22. Evan A, Huser J, Bengele HH, Alexander EA (1980) The effect of alterations in dietary potassium on collecting system morphology in the rat. Lab Invest 42: 668–675PubMedGoogle Scholar
  23. Farquhar MG (1981) The glomerular basement membrane. A selective macromolecular filter. In: Hay ED (ed) Cell biology of extracellular matrix. Plenum, New York, pp 335–378CrossRefGoogle Scholar
  24. Farquhar MG, Palade GE (1962) Functional evidence for the existence of a third cell type in the renal glomerulus. Phagocytosis of filtration residues by a distinctive “third” cell. J Cell Biol 13: 55–87PubMedCrossRefGoogle Scholar
  25. Farquhar MG, Wissig SL, Palade GE (1961) Glomerular permeability: I. Ferritin transfer across the normal glomerular capillary wall. J Exp Med 113: 47–66PubMedCrossRefGoogle Scholar
  26. Foidart J, Sraer J, DeLarue F, Mahieu P, Ardaillou R (1980) Evidence for mesangial glomerular receptors for angiotensin II linked to mesangial cell contractility. FEBS Lett 121: 333–339PubMedCrossRefGoogle Scholar
  27. Forssmann WG, Taugner R (1977) Studies on the juxtaglomerular apparatus. V. The juxtaglomerular apparatus in Tupaia with special reference to intercellular contacts. Cell Tissue Res 177: 291–305PubMedGoogle Scholar
  28. Frömter E (1979) Solute transport across epithelia: what can we learn from micropuncture studies in kidney tubules? J Physiol (Lond) 288:1–31 (The Feldberg lecture 1976)Google Scholar
  29. Garg LC Knepper MA, Burg MB (1981) Mineralocorticoid effects on Na-K-ATPase in individual nephron segments. Am Physiol 240 9: F536–F544Google Scholar
  30. Goormaghtigh N (1939) Existence of an endocrine gland in the media of the renal arterioles. Proc Soc Exp Biol Med 42: 688–689Google Scholar
  31. Gorgas K (1978) Structure and innervation of the juxtaglomerular apparatus of the rat. Adv Anat Embryol Cell Biol 54:3–83 (English abstract)PubMedGoogle Scholar
  32. Grantham JJ (1971) Mode of water transport in mammalian renal collecting tubules. Fed Proc 30: 14–21PubMedGoogle Scholar
  33. Grantham JJ, Burg MB (1966) Effect of vasopressin and cyclic AMP on permeability of isolated collecting tubules. Am J Physiol 211: 255–259PubMedGoogle Scholar
  34. Hansen GP, Tisher CC, Robinson RR (1980) Response of the collecting duct to disturbance of acid-base and potassium balance. Kidney Int 17: 326–337PubMedCrossRefGoogle Scholar
  35. Helmchen UE (1980) Die Zahl der Mesangiumzellen in einem normalen Glomerulum der Rattenniere: eine dreidimensionale elektronenoptische Analyse. Inaugural dissertation, Tübingen UniversityGoogle Scholar
  36. Hoyer JR, Sisson SP, Vernier RL (1979) Tamm-Horsfall glycoprotein. Ultrastructural immunoperoxidase localization in rat kidney. Lab Invest 41: 168–173PubMedGoogle Scholar
  37. Imai M (1977) Function of the thin ascending limb of Henle of rats and hamsters perfused in vitro. Am J Physiol 232: F201–F209PubMedGoogle Scholar
  38. Imai M, Hayashi M, Araki M, Tabei K (1984) Function of the thin limb of Henle’s loop. In: Robinson RR (ed) Nephrology, vol 1. Springer, Berlin Heidelberg New York Tokyo, pp 196–207CrossRefGoogle Scholar
  39. Jamison RL, Kriz W (1982) Urinary concentrating mechanism. Structure and function. Oxford University Press. New YorkGoogle Scholar
  40. Kaissling B (1980) Ultrastructural organization of the transition from the distal nephron to the collecting duct in desert rodent Psammomys obesus. Cell Tissue Res 212: 475–495PubMedCrossRefGoogle Scholar
  41. Kaissling B (1982) Structural aspects of adaptive changes in renal electrolyte excretion. Am J Physiol 243: F211–F226PubMedGoogle Scholar
  42. Kaissling B, Kriz W (1979) Structural analysis of the rabbit kidney. Adv Anat Embryol Cell Biol 56: 1–123PubMedCrossRefGoogle Scholar
  43. Kaissling B, Kriz W (1982) Variability of intercellular spaces between macula densa cells. A transmission electron microscopic study in rabbits and rats. Kidney Int 12: S9–S17Google Scholar
  44. Kaissling B, Le Hir M (1985) Anpassung distaler Tubulussegmente an Änderungen im Elektrolythaushalt. Acta Histochem [Suppl] (Jena) 31: 185 - 192Google Scholar
  45. Kaissling B. Peter S, Kriz W (1977) The transition of the thick ascending limb of Henle’s loop into the distal convoluted tubule in the nephron of the rat kidney. Cell Tissue Res 182: 111–118PubMedCrossRefGoogle Scholar
  46. Kaissling B, Bachmann S, Kriz W (1985) Structural adaptation of the distal convoluted tubule to prolonged furosemide treatment. Am J Physiol 248 17: F374–F381PubMedGoogle Scholar
  47. Karnovsky MJ (1979) The structural bases for glomerular filtration. In: Jacob Churg et al. (ed) Kidney disease - present status. Williams and Wilkins, Baltimore, pp 1–41 (IAP monograph 20 )Google Scholar
  48. Katz AI, Doucet A, Morel F (1979) Na-K-ATPase activity along the rabbit, rat and mouse nephron. Am J Physiol 237: F114–F120PubMedGoogle Scholar
  49. Keijaschki D, Noronha-Blob L, Sacktor B, Farquhar MG (1984) Microdomains of distinctive glycoprotein composition in the kidney proximal tubule brush border. J Cell Biol 98: 1505–1513CrossRefGoogle Scholar
  50. Kirk KL, DiBona DR, Schafer JA (1984a) Morphologic response of the rabbit cortical collecting tubule to peritubular hypotonicity: quantitative examination with differential interference contrast microscopy. J Membr Biol 79: 53–64PubMedCrossRefGoogle Scholar
  51. Kirk KL, Bell D, Barfuss DW, Ribadeneira M (1985) Direct visualization of the isolated and perfused macula densa. Am J Physiol 248 17: F890–F894Google Scholar
  52. Kriz W (1967) Der architektonische und funktionelle Aufbau der Rattenniere. Z Zellforsch Mikrosk Anat 82: 495–535PubMedCrossRefGoogle Scholar
  53. Kriz W, Bankir L (1982) ADH-induced changes in the epithelium of the thick ascending limb in Brattleboro rats with hereditary hypothalamic diabetes insipidus. Ann NY Acad Sci 394: 424–432PubMedCrossRefGoogle Scholar
  54. Kriz W, Schnermann J, Dieterich HJ (1972) Differences in the morphology of descending limbs of short and long loops of Henle in the rat kidney. In: Wirz H, Spinelli F (eds) Recent advances in renal physiology. Karger, Basel, pp 140–144Google Scholar
  55. Kriz W, Kaissling B, Psczolla M (1978) Morphological characterization of the cells in Henle’s loop and the distal tubule. In: Vogel HG, Ullrich KJ (eds) New aspects of renal function, vol 6. Excerpta Medica, Amsterdam, pp 67–78Google Scholar
  56. Kriz W, Schiller A, Taugner R (1981) Freeze-fracture studies on the thin limbs of Henle’s loop in Psammomys obesus. Am J Anat 162: 23–33PubMedCrossRefGoogle Scholar
  57. Latta H, Maunsbach AB (1962) The juxtaglomerular apparatus as studied electron microscopically. J Ultrastruct Res 6: 547–561PubMedCrossRefGoogle Scholar
  58. Laurie GW, Leblond CP, Inoue S, Martin GR, Chung A (1984) Fine structure of the glomerular basement membrane and immunolocalization of five basement membrane components to the lamina densa (basal lamina) and its extensions in both glomeruli and tubules of the rat kidney. Am J Anat 169: 463–481PubMedCrossRefGoogle Scholar
  59. Le Hir M, Kaissling B, Dubach UC (1982) Distal tubular segments of the rabbit kidney after adaptation to altered Na+ and K+ intake. Changes in Na-K-ATPase activity. Ceil Tissue Res 224: 493–504Google Scholar
  60. Lönnerholm G, Wistrand PJ (1984) Carbonic anhydrase in the human kidney: a histochemical and immunocytochemical study. Kidney Int 25: 886–898PubMedCrossRefGoogle Scholar
  61. Madsen KM, Tisher CC (1983) Cellular response to acute respiratory acidosis in rat medullary collecting duct. Am J Physiol 245 14: F670–F679PubMedGoogle Scholar
  62. Madsen KM, Tisher CC (1984) Response of intercalated cells of rat outer medullary collecting duct to chronic metabolic acidosis. Lab Invest 51: 268–276PubMedGoogle Scholar
  63. Marsh DJ (1970) Solute and water flows in thin limbs of Henle’s loop in the hamster kidney. Am J Physiol 218: 824–831PubMedGoogle Scholar
  64. Maunsbach AB (1966) Observations on the segmentation of the proximal tubule in the rat kidney. Comparison of results from phase contrast, fluorescence and electron microscopy. J Ultrastruct Res 16: 239–258PubMedCrossRefGoogle Scholar
  65. Maunsbach AB (1973) Ultrastructure of the proximal tubule. In: Orloff J, Berliner RW (eds) Handbook of physiology, vol 8. American Physiological Society, Washington, pp 31–79Google Scholar
  66. Morel F, Chabardes D, Imbert-Teboul M, Le Bouffant F, Hus-Citharel A, Montegut M (1982) Multiple hormonal control of adenylate cyclase in distal segments of the rat kidney. Kidney Int [Suppl] 11: S55–S62Google Scholar
  67. Neiss WF (1981) Morphogenese und Histogenese des Verbindungsstücks in der Rattenniere. Acta Anat 111: 105–106 (abstract)Google Scholar
  68. Peter S, Lazar J, Gross F, Forssmann WG (1974) Studies on the juxtaglomerular apparatus: II. Quantitative morphology after adrenalectomy. Cell Tissue Res 151: 457–469PubMedCrossRefGoogle Scholar
  69. Pricam C, Humbert F, Perrelet A, Orci L (1974) A freeze-etch study of the tight juctions of the rat kidney tubules. Lab Invest 30: 286–291PubMedGoogle Scholar
  70. Rastegar A, Biemesderfer D, Kashgarian M, Hayslett JP (1980) Changes in membrane surfaces of collecting duct cells in potassium adaptation. Kidney Int 18: 293–301PubMedCrossRefGoogle Scholar
  71. Reeves WH, Kanvar YS, Farquhar MG (1980) Assembly of the glomerular filtration surface. Differentiation of anionic sites in glomerular capillaries of newborn rat kidney. J Cell Biol 85: 735–753PubMedCrossRefGoogle Scholar
  72. Rodewald R, Karnovsky MJ (1974) Porous substructure of the glomerular slit diaphragm in the rat and mouse. J Cell Biol 60: 423–433PubMedCrossRefGoogle Scholar
  73. Roesinger B, Schiller A, Taugner R (1978) A freeze-fracture study of tight junctions in the pars convoluta and pars recta of the renal proximal tubule. Cell Tissue Res 186: 121–133PubMedCrossRefGoogle Scholar
  74. Roll FJ, Madri J A, Albert J, Furthmayr H (1980) Codistribution of collagen types IV and AB2 in the basement membranes and mesangium of the kidney. An immunoferritin study of ultrathin frozen sections. J Cell Biol 85: 597–616PubMedCrossRefGoogle Scholar
  75. Rostgaard J, Thuneberg L (1972) Electron microscopical observations on the brush border of proximal tubule cells of mammalian kidney. Z Zellforsch 132: 473–496PubMedCrossRefGoogle Scholar
  76. Rouiller C, Orci L (1971) The structure of the juxtaglomerular complex. In: Rouiller C, Muller AF (eds) The kidney: morphology, biochemistry, physiology, vol 4. Academic, New York, pp 1–80Google Scholar
  77. Scherzer P, Wald H, Czaczkes JW (1985) Na-K-ATPase in isolated rabbit tubules after unilateral nephrectomy and Na+ loading. Am J Physiol 248 17: F565–F573Google Scholar
  78. Schnabel E, Kriz W (1984) Morphometry studies of the extraglomerular mesangial cell field in volume expanded and volume depleted rats. Anat Embryol (Berl) 170: 217–222CrossRefGoogle Scholar
  79. Schwartz GJ, Al-Awqati Q (1985) Carbon dioxide causes exocytosis of vesicles containing H+ pumps in isolated perfused proximal and collecting tubules. J Clin Invest 75: 1638–1644PubMedCrossRefGoogle Scholar
  80. Schwartz MM. Venkatachalam MA (1974) Structural differences in thin limbs of Henle: physiological implications. Kidney Int 6: 193–208PubMedCrossRefGoogle Scholar
  81. Schwartz MM, Karnovsky MJ, Venkatachalam MA (1979) Regional membrane specialization in the thin limbs of Henle’s loop as seen by freeze-fracture electron microscopy. Kidney Int 16: 577–589PubMedCrossRefGoogle Scholar
  82. Sikri KL, Foster CL (1981) Light and electron microscopical observations on the macula densa of the Syrian hamster kidney. J Anat 132: 57–69PubMedGoogle Scholar
  83. Silbernagel S (1985) Amino acids and oligopeptides. In: Seldin DW, Giebisch G (eds) The kidney: physiology and pathophysiology. Raven. New York, pp 1677–1701Google Scholar
  84. Stanton B, Janzen A, Klein-Robbenhaar G, DeFronzo R, Giebisch G, Wade J (1985) Ultrastructure of rat initial collecting tubule. Effects of adrenal corticosteroid treatment. J Clin Invest 75: 1327–1334PubMedCrossRefGoogle Scholar
  85. Stetson DL, Wade JB, Giebisch G (1980) Morphologic alterations in the rat medullary collecting duct following potassium depletion. Kidney Int 17: 45–56PubMedCrossRefGoogle Scholar
  86. Taugner R, Schiller A, Kaissling B, Kriz W (1978) Gap junctional coupling between the JGA and the glomerular tuft. Cell Tissue Res 186: 279–285PubMedCrossRefGoogle Scholar
  87. Taugner R, Mannek E, Nobiling R, Biihrle CP, Hackenthal E, Ganten D, Inagami T, Schröder H (1984) Coexistence of renin and angiotensin II in epithelioid ceil secretory granules of rat kidney. Histochemistry 81: 39–45PubMedCrossRefGoogle Scholar
  88. Trenchev P, Dorling J, Webb J, Holborrow EJ (1976) Localization of smooth muscle-like contractile proteins in kidney by immunoelectron microscopy. J Anat 121: 85–95PubMedGoogle Scholar
  89. Vasmant D, Maurice M, Feldmann G (1984) Cytoskeleton ultrastructure of podocytes and glomerular endothelial cells in man and in the rat. Anat Rec 210: 17–24PubMedCrossRefGoogle Scholar
  90. Verlander JW, Madsen KM, Tisher CC (1985) Two populations of intercalated ceils exist in the cortical collecting duct of the rat. Clin Res 33:501 AGoogle Scholar
  91. Wade JB, O’Neil RG, Pryor JL, Boulpaep EL (1979) Modulation of cell membrane area in renal collecting tubules by corticosteroid hormones. J Cell Biol 81P:439–445CrossRefGoogle Scholar
  92. Zalups RK, Stanton BA, Wade JB, Giebisch G (1985) Structural adaptation in initial collecting tubule following reduction in renal mass. Kidney Int 27: 636–642PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • S. Bachmann
    • 1
  • T. Sakai
    • 2
  • W. Kriz
    • 1
  1. 1.Institute of AnatomyUniversity of HeidelbergHeidelbergGermany
  2. 2.Department of Anatomy, Faculty of MedicineUniversity of TokyoTokyoJapan

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