Abstract
The life cycle of the unicellular flagellate, Trypanosoma brucei, in the mammalian host and the tsetse fly proceeds through a series of morphological states which are accompanied by profound alterations in ultrastructure and metabolism. The developmental step most extensively studied is the differentiation of bloodstream to procyclic trypamostigotes which occurs in the midgut of the fly after uptake with the blood meal. This transition, also termed transformation, is initiated in the mammal by the differentiation of dividing bloodstream forms with slender morphology to non-dividing, stumpy cells. The latter cells are considered to convert most readily to dividing procyclic cells in the midgut of the fly. Several culture media have been devised which allow a more or less synchronous transformation of either pleomorphic populations of bloodstream forms or monomorphic populations which have a uniformly slender morphology and arise from pleomorphic strains by frequent passage in rodents.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bangs JD, Andrews NW, Hart GW, Englund PT (1986) Post translational modification and intracellular transport of a trypanosome variant surface glycoprotein. J Cell Biol 103: 255–263.
Borst P (1986) Discontinuous transcription and antigenic variation in trypanosomes. Ann Rev Biochem 55: 701–732.
Brun R, Jenni L (1985) Cultivation of African and South American trypanosomes of medical and veternary importance. Br Med Bull 41: 122–129.
Brun R, Schinenberger M (1981) Stimulating effect of citrate and cis-aconitate on the transformation of Trypanosoma brucei bloodstream forms to procyclic forms in vitro. Z Parasitkd 66: 17–24.
Bülow R, Overath P (1985) Synthesis of a hydrolase for the membrane-form variant surface glycoprotein is repressed during transformation of Trypanosoma brucei. FEBS Lett. 187: 105–110.
Bülow, R, Overath P (1986) Purification and characterization of the membrane-form variant surface glycoprotein hydrolase of Trypanosoma brucei. J Biol Chem 261: 11918–11923.
Cardoso de Almeida ML, Turner MJ (1983) The membrane form of variant surface glycoproteins of Trypanosoma brucei. Nature 302: 349–352.
Czichos J, Nonnengaesser C, Overath P (1986) Trypanosoma brucei: cis-Aconitate and temperature reduction as triggers of synchronous transformation of bloodstream to procyclic trypomastigotes in vitro. Exp Parasitol 62: 283–291.
Ehlers B, Czichos J, Overath P (1986) RNA turnover in Trypanosoma brucei. Mol Cell Biol submitted for publication.
Fairlamb AH, Opperdoes FR (1986) Cabohydrate metabolism in African trypanosomes, with special reference to the glycosome. In: Morgan MJ (ed) Carbohydrate metabolism in cultured cells. Plenum Publishing Corporation, New York.
Ferguson MAJ, Low MG, Cross GAM (1985a) Glycosyl-sn-1, 2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein. J Biol Chem 260: 14547–14555.
Ferguson MAJ, Haldar K, Cross GAM (1985b) Trypanosoma brucei variant surface glycoprotein has an sn-1, 2-dimyristylglycerol membrane anchor at its C00H terminus. J Biol Chem 260: 4963–4968.
Haston WS (1975) Studies on the early development of Trypanosoma brucei in the vector Glossina morsitans. PhD thesis, Glasgow University.
Overath P, Czichos J, Stock U, Nonnengaesser C (1983) Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma brucei. EMBO J 2: 1721–1728.
Overath P, Czichos J, Haas C (1986) The effect of citrate/cis-aconitäte on oxidative metabolism during transformation of Trypanosoma brucei. Eur J Biochem 160: 175–182.
Shapiro SJ (1986) Trypanosomabrucei: Release of variant surface glycoprotein during the parasite life cycle. Exp Parasitol 61:432–437.
Vickerman K (1985) Developmental cycles and biology of pathogenic trypanosomes. Br Med Bull 41: 105–114.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Overath, P., Bülow, R., Czichos, J., Ehlers, B. (1987). Differentiation of Trypanosoma brucei from Bloodstream to Procyclic Trypomastigotes. In: Chang, KP., Snary, D. (eds) Host-Parasite Cellular and Molecular Interactions in Protozoal Infections. NATO ASI Series, vol 11. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72840-2_5
Download citation
DOI: https://doi.org/10.1007/978-3-642-72840-2_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-72842-6
Online ISBN: 978-3-642-72840-2
eBook Packages: Springer Book Archive