Differentiation of Trypanosomabrucei from Bloodstream to Procyclic Trypomastigotes
The life cycle of the unicellular flagellate, Trypanosoma brucei, in the mammalian host and the tsetse fly proceeds through a series of morphological states which are accompanied by profound alterations in ultrastructure and metabolism. The developmental step most extensively studied is the differentiation of bloodstream to procyclic trypamostigotes which occurs in the midgut of the fly after uptake with the blood meal. This transition, also termed transformation, is initiated in the mammal by the differentiation of dividing bloodstream forms with slender morphology to non-dividing, stumpy cells. The latter cells are considered to convert most readily to dividing procyclic cells in the midgut of the fly. Several culture media have been devised which allow a more or less synchronous transformation of either pleomorphic populations of bloodstream forms or monomorphic populations which have a uniformly slender morphology and arise from pleomorphic strains by frequent passage in rodents.
KeywordsSurface Coat Trypanosoma Brucei Bloodstream Form Variant Surface Glycoprotein Flagellar Pocket
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