Entamoeba Histolytica: Activation and Release of Membrane Dense Bodies

  • A. Martinez-Palomo
  • I. Meza
  • B. Chávez
  • J. L. Rosales-Encina
  • M. L. Muñoz
  • A. González-Robles
  • M. Rojkind
Part of the NATO ASI Series book series (volume 11)

Abstract

The protozoan parasite Entamoeba histolytica is the etiological agent of invasive amebiasis, a common human disease in certain developing countries. The requirement of close contact between the parasite and target cells for cytolysis to occur (Ravdin & Guerrant 1981; Martínez-Palomo, 1982) and the isolation of amebic proteins capable of forming aqueous pores upon their insertion into the plasma membrane of target eukaryotic cells (Lynch et al. 1982; Young et al. 1982) suggest that membrane interactions have an important role in the powerful cytolytic activity of pathogenic amebas. Tissue invasion may be facilitated by the collagenolytic and proteolytic activities described in this protozoan (Muñoz et al. 1982; Gadasi & Kessler 1983; Keene et al. 1986).

Keywords

Sucrose EDTA Fractionation Dehydration Mannitol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aley SB, Scott WA, Cohn ZJ (1980) Plasma membrane of Entamoeba histolytica. J Exp Med 152: 391–404PubMedCrossRefGoogle Scholar
  2. Diamond LS, Harlow DR, Cunnick CC (1978) A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans Roy Soc Trop Med Hyg 72s431–432Google Scholar
  3. Gadasi H, Kessler E (1983) Correlation of virulence and collagenolytic activity in Entamoeba histolytica. Infec Immun 39: 528–531Google Scholar
  4. Keene WE, Petitt MG, Allen S, McKerrow JH (1986) The major neutral proteinase of Entamoeba histolytica. J Exp Med 163: 536–549PubMedCrossRefGoogle Scholar
  5. Lynch EC, Rosenberg IM, Gitler C (1982) An ion-channel forming protein produce by Entamoeba histolytica. Euro Mol Biol Organ J 1: 801 - 804Google Scholar
  6. Martinez-Palomo A (1982) The Biology of Entamoeba histolytica. Research Studies Press/John Wiley, ChichesterGoogle Scholar
  7. Muñoz ML, Calderón J, Rojkind M (1982) The collagenase of Entamoeba histolytica. J Exp Med 155: 42–51PubMedCrossRefGoogle Scholar
  8. Ravdin JL, Guerrant RL (1981) Role of adherence in cytopathogenic mechanisms of Entamoeba histolytica. J Clin Invest 68: 1305–1313PubMedCrossRefGoogle Scholar
  9. Rondanelli EG, Osculati F, Gerna G, Fiori GP (1965) Ricerche microelettroniche su Entamoeba invaflens. Boll Ist Sieroter Milan 44: 218–239PubMedGoogle Scholar
  10. Sobota A (1985) Subplasmalemmal calcium-binding microregions in Acanthamoeba. J Cell Sci 79: 217–235PubMedGoogle Scholar
  11. Young JD, Young TM, Lu LP, Unkless JC,Cohn Z (1982) Characterization of a membrane pore-forming protein from Entamoeba histolytica. J Exp Med 156: 1677–1690Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • A. Martinez-Palomo
    • 1
  • I. Meza
    • 1
  • B. Chávez
    • 1
  • J. L. Rosales-Encina
    • 1
  • M. L. Muñoz
    • 1
  • A. González-Robles
    • 1
  • M. Rojkind
    • 1
  1. 1.Center for Research and Advanced StudiesNational Polytechnical InstituteMexico, D.F.Mexico

Personalised recommendations