Studies on the Plasmodium Falciparum 195,000 Molecular Mass Merozoite Surface Antigen Precursor

  • A. A. Holder
  • Y. Hillman
  • S. C. Nicholls
  • L. S. Davey
Conference paper
Part of the NATO ASI Series book series (volume 11)

Abstract

One of the proteins synthesized by the asexual blood stage of malaria which has been studied in some detail is the precursor to the major merozoite surface antigens (PMMSA), a glycoprotein that is synthesized throughout schizogony and present on the merozoite surface in processed form (Holder & Freeman, 1982, 1984; Howard et al., 1985). Complete or partial nucleotide sequences of the gene for this protein from a number of Plasmodium falciparum strains have been reported (Holder, et al., 1985; Mackay et al., 1985; Cheung et al., 1985; Weber et al,1986). Some features of the protein and its gene will be discussed here, in particular studies we have performed with a West African (Wellcome) strain of the parasite.

Keywords

Codon Recombination Glycine Cysteine Electrophoresis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Borst P, Cross GAM (1982) Molecular basis for trypanosome antigenic variation. Cell 29: 291–303PubMedCrossRefGoogle Scholar
  2. Cheung A, Shaw AR, Leban J, Perrin LH (1985) Cloning and expression in Escherichia coli of a surface antigen of Plasmodium falciparum merozoites. EMBO J 4: 1007–1012PubMedGoogle Scholar
  3. Doolittle RF (1981) Similar amino acid sequences: chance or common ancestry? Science 214: 149–159PubMedCrossRefGoogle Scholar
  4. Freeman RR, Holder AA (1983) Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83,000-mol wt form expressed on the surface of Plasmodium falciparum merozoites. J exp Med 158: 1647–1653PubMedCrossRefGoogle Scholar
  5. Holder AA, Freeman RR(1982) Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody. J exp Med 156: 1528–1538Google Scholar
  6. Holder AA, Freeman RR (1984) The three major antigens on the surface of Plasmodium falciparum merozoites are derived from a single high molecular weight precursor. J exp Med 160: 624–629PubMedCrossRefGoogle Scholar
  7. Holder AA, Lockyer MJ, Odink KG, Sandhu JS, Riveros-Moreno V, Nicholls SC, Hillman Y, Davey LS, Tizard MLV, Schwarz RT, Freeman RR (1985) Primary structure of the precursor to the three major surface antigens of Plasmodium falciparum merozoites. Nature 317: 270–273PubMedCrossRefGoogle Scholar
  8. Holder AA, Sandhu JS, Hillman Y, Davey LS, Nicholls SC, Lockyer MJ (1987) Processing of the precursor to the major merozoite surface antigens of Plasmodium falciparum, (submitted)Google Scholar
  9. Howard RF, Stanley HA, Campbell GH, Langreth SG, Reese RT (1985) Two Plasmodium falciparum merozoite surface polypeptides share epitopes with a single M 185,000 parasite glycoprotein. Mol Biochem Parasitol 17: 61–77PubMedCrossRefGoogle Scholar
  10. Lyon JA, Geller RH, Haynes JD, Chulay JD, Weber JL(1986) Epitope map and processing scheme for the 195,000-dalton surface glycoprotein of Plasmodium falciparum merozoites deduced from cloned overlapping segments of the gene. Proc Natl Acad Sci USA 83: 2989–2993Google Scholar
  11. Mackay M, Goman M, Bone N, Hyde JE, Scaife J, Certa U, Stunnenberg H, Bujard H (1985) Polymorphism of the precursor for the major surface antigens of Plasmodium falciparum merozoites: studies at the genetic level. EMBO J 4: 3823–3829PubMedGoogle Scholar
  12. McBride JS, Newbold CI, Anand R (1985) Polymorphism of a high molecular weight schizont antigen of the human malaria parasite Plasmodium falciparum. J Exp Med 161: 160–180PubMedCrossRefGoogle Scholar
  13. Schwarz RT, Riveros-Moreno V, Lockyer MJ, Nicholls SC, Davey LS, Hillman Y, Sandhu JS, Freeman RR, Holder AA(1986) Structural diversity of the major antigen of Plsmodium falciparum merozoites. Mol Cell Biol 6: 964–968Google Scholar
  14. Staden R (1982) An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucl Acids Res 10: 2951–2961PubMedCrossRefGoogle Scholar
  15. Steers E, Cuatrecasas P, Pollard HB (1971) The purification of ß-galactosidase from Escherichia coli by affinity chromatography. J Biol Chem 246: 196–200PubMedGoogle Scholar
  16. Weber JL, Leininger WM, Lyon JA (1986) Variation in the gene encoding a major merozoite surface antigen of the human malaria parasite Plasmodium falciparum. Nucl Acids Res 14: 3311–3323PubMedCrossRefGoogle Scholar
  17. Winther MD, Allen G, Bomford RH, Brown F (1986) Bacterially expressed antigenic peptide from foot-and-mouth disease virus capsid elicits variable immunologic responses in animals. J Immunol 136: 1835–1840PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • A. A. Holder
    • 1
  • Y. Hillman
    • 1
  • S. C. Nicholls
    • 1
  • L. S. Davey
    • 1
  1. 1.Department of Molecular BiologyWellcome BiotechBeckenham, KentUK

Personalised recommendations