Interaction of Leishmania and Membrane-Stabilizing Drugs with Human Phagocytic Cells
Leishmaniasis inflicts more than one million people in the world and causes over 1000 deaths per year. Therapy of these patients poses a serious problem. Pentavalent antimonials at higher doses exhibit considerable toxicity, some patients with visceral leishmaniasis become unresponsive to conventional doses of antimonials, and the usual second line drugs such as pentamidine and amphotericin B are also toxic and difficult to administer. In order to devise more effective ways to control the disease, there is a great need for better understanding of the host-parasite interactions. Identification and characterization of the parasite products which are involved in attraction of the parasite by the host cells, and in binding to and ingestion of the parasite by these cells will be of particular interest in this context.
KeywordsToxicity Serotonin Stein Dine Amphotericin
Unable to display preview. Download preview PDF.
- Bray RS (1983) Leishmania: Chemotaxic responses of promastigotes and macrophages invitro. J. of Protozool. 30(2): 322–329.Google Scholar
- Chang CS, Chang KP (1986) Monoclonal affinity purification of a leishmania membrane glycoprotein and its inhibition of leishmania-macrophage binding. Proc. Nat. Acad. Sci. 83: 100–104.Google Scholar
- Heron DS, Shinitzky M, Hershkowitz M, Samuel D (1980) Lipid fluidity markedly modulates the binding of serotonin to mouse brain membranes. Proc. Nat. Acad. Sci. 77: 7463–7467.Google Scholar
- Ogiso T, Iwaki M, Mori K (1981) Fluidity of human erythrocyte membrane and effect of chlorpromazine on fluidity and phase separation of membrane. Biochem. Biophys. Acat. 649: 325–335.Google Scholar
- Rechnitzer C, Kristiansen JE, Kharazmi A (1985) In vitro modulation of human neutrophil chemotaxis by cis(Z)- and trans(E)- clopenthixol and chlorpromazine. Acta Pathol. Microbiol. Immunol. Sec. C. 93: 199–203.Google Scholar
- Silverstein SC (1977) Endocytic uptake of particles by mononuclear phagocytes and the penetration of obligate intracellular parasites. Am. J. Trop. Med. Hyg. 26(6) suppl.: 161–168.Google Scholar
- Smith RJ, Bowmann BJ, Iden SI (1981) Effects of trifluoperazine on human neutrophil function. Immunol. 44: 677–684.Google Scholar
- Suda T, Shimizu D, Maeda N, Shiga T (1981) Decreased viscosity of human erythrocyte suspension induced by chlorpromazine and isoxsusprine. Biochem. Pharmacol. 30: 2057–2064.Google Scholar
- Sorensen AL, Kharazmi A, Nielsen H (1986) Leishmania promasti- gote sonicate exhibits chemotactic activity for human neutrophils and monocytes. Submitted.Google Scholar
- Trouet A, Tulkens P, Schneider YJ (1980) Subcellular localization of infectious agents: Pharmacological and pharmacokinetic implications. In: The Host Invader Interplay, ed. Van den Bossche, North Holland H. p. 31–44.Google Scholar