Interaction of Leishmania and Membrane-Stabilizing Drugs with Human Phagocytic Cells

  • Arsalan Kharazmi
Conference paper
Part of the NATO ASI Series book series (volume 11)


Leishmaniasis inflicts more than one million people in the world and causes over 1000 deaths per year. Therapy of these patients poses a serious problem. Pentavalent antimonials at higher doses exhibit considerable toxicity, some patients with visceral leishmaniasis become unresponsive to conventional doses of antimonials, and the usual second line drugs such as pentamidine and amphotericin B are also toxic and difficult to administer. In order to devise more effective ways to control the disease, there is a great need for better understanding of the host-parasite interactions. Identification and characterization of the parasite products which are involved in attraction of the parasite by the host cells, and in binding to and ingestion of the parasite by these cells will be of particular interest in this context.


Visceral Leishmaniasis Chemotactic Activity Leishmania Species Leishmania Parasite Lipid Fluidity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ardehali SM, Khoubyar K, Rezai HR (1979) Studies on the effect of the antiphagocytic agent cytochalasin B on the Leishmania- macrophage interaction. Acta Tropica 36: 15–21.PubMedGoogle Scholar
  2. Blackwell JM (1985) Receptors and recognition mechanisms of Leishmania species. Trans. Roy. Soc. Trop. Med. Hyg. 79: 606–612.PubMedCrossRefGoogle Scholar
  3. Blackwell JM, Ezekowitz RAB, Roberts MB, Channon JY, Sim RB, Gordon S (1985) Macrophage complement and lectin-like receptors bind Leishmania in the absence of serum. J. Exp. Med. 162: 324–331.PubMedCrossRefGoogle Scholar
  4. Bray RS (1983) Leishmania: Chemotaxic responses of promastigotes and macrophages invitro. J. of Protozool. 30(2): 322–329.Google Scholar
  5. Chang CS, Chang KP (1986) Monoclonal affinity purification of a leishmania membrane glycoprotein and its inhibition of leishmania-macrophage binding. Proc. Nat. Acad. Sci. 83: 100–104.Google Scholar
  6. Handman E, Goding JW (1985) The Leishmania receptor for macrophages is a lipid-containing glycoconjugate. EMBO J. 4: 329–336.PubMedGoogle Scholar
  7. Herman R (1980) Cytophilic and opsonic antibodies in visceral leishmaniasis in mice. Infect. Immun. 28: 585–593.PubMedGoogle Scholar
  8. Heron DS, Shinitzky M, Hershkowitz M, Samuel D (1980) Lipid fluidity markedly modulates the binding of serotonin to mouse brain membranes. Proc. Nat. Acad. Sci. 77: 7463–7467.Google Scholar
  9. Ogiso T, Iwaki M, Mori K (1981) Fluidity of human erythrocyte membrane and effect of chlorpromazine on fluidity and phase separation of membrane. Biochem. Biophys. Acat. 649: 325–335.Google Scholar
  10. Rechnitzer C, Kristiansen JE, Kharazmi A (1985) In vitro modulation of human neutrophil chemotaxis by cis(Z)- and trans(E)- clopenthixol and chlorpromazine. Acta Pathol. Microbiol. Immunol. Sec. C. 93: 199–203.Google Scholar
  11. Silverstein SC (1977) Endocytic uptake of particles by mononuclear phagocytes and the penetration of obligate intracellular parasites. Am. J. Trop. Med. Hyg. 26(6) suppl.: 161–168.Google Scholar
  12. Smith RJ, Bowmann BJ, Iden SI (1981) Effects of trifluoperazine on human neutrophil function. Immunol. 44: 677–684.Google Scholar
  13. Suda T, Shimizu D, Maeda N, Shiga T (1981) Decreased viscosity of human erythrocyte suspension induced by chlorpromazine and isoxsusprine. Biochem. Pharmacol. 30: 2057–2064.Google Scholar
  14. Sorensen AL, Kharazmi A, Nielsen H (1986) Leishmania promasti- gote sonicate exhibits chemotactic activity for human neutrophils and monocytes. Submitted.Google Scholar
  15. Trouet A, Tulkens P, Schneider YJ (1980) Subcellular localization of infectious agents: Pharmacological and pharmacokinetic implications. In: The Host Invader Interplay, ed. Van den Bossche, North Holland H. p. 31–44.Google Scholar
  16. Zenian A (1980) Leishmania tropica: Biochemical aspects of promastigote attachment to macrophages in vitro. Exp. Parasitol. 51: 175–187.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • Arsalan Kharazmi
    • 1
  1. 1.State Serum Institute, Department of Clinical MicrobiologyRigshospitaletCopenhagen NDenmark

Personalised recommendations