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The Role of Metal Ions in Bacteriorhodopsin Function

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Primary Processes in Photobiology

Part of the book series: Springer Proceedings in Physics ((SPPHY,volume 20))

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Abstract

The literature on the simultaneous dissociation of an acid of pKa ~8.6–10 and the protonated Schiff base and a possible coupling mechanism is reviewed. The effect of metal cations on the color of retinal and the photocycle is also summarized in light of this coupling. Studies using Eu3+ to probe the ion binding sites in bacteriorhodopsin by following the spectroscopy and kinetics of its emission are reported. It is shown that the Eu3+ binding constants for the different sites appear quite similar, and that the total intensity of the emission increases linearly with the amount of added Eu3+. A model is presented incorporating these results, explaining how the retinal color and extent of deprotonation in the photocycle can change sigmoidally with the amount of added cations, based on a requirement that more than one metal ion must be bound to allow these processes to occur.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, 90024, USA

    T. C. Corcoran, E. S. Awad & M. A. El-Sayed

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  1. T. C. Corcoran
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  2. E. S. Awad
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  3. M. A. El-Sayed
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Editor information

Editors and Affiliations

  1. Department of Physics, Faculty of Science, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

    Takayoshi Kobayashi

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© 1987 Springer-Verlag Berlin Heidelberg

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Corcoran, T.C., Awad, E.S., El-Sayed, M.A. (1987). The Role of Metal Ions in Bacteriorhodopsin Function. In: Kobayashi, T. (eds) Primary Processes in Photobiology. Springer Proceedings in Physics, vol 20. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72835-8_24

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  • DOI: https://doi.org/10.1007/978-3-642-72835-8_24

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72837-2

  • Online ISBN: 978-3-642-72835-8

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