Activity of Bacteriorhodopsin in the Presence of a Large pH Gradient

  • T. Kouyama
  • A. N. Kouyama
  • A. Ikegami
Conference paper
Part of the Springer Proceedings in Physics book series (SPPHY, volume 20)

Abstract

Bacteriorhodopsin (bR) is a membrane protein found in Halobacterium halobium. Due to the retinal chromophore bound to the apoprotein via a protonated Schiff base linkage, bR shows a strong absorption band in the visible region. Light excitation of the chromophore initiates a photochemical cycle of bR, through which protons are actively translocated from the cytoplasmic side to the outside, thereby generating a pH gradient across the plasma membrane [1]. It has been shown that formation of a yellow intermediate M412 is accompanied with deprotonation of the Schiff base [2], and it has been widely accepted that its deprotonation (and subsequent reprotonation) is coupled with a unidirectional flow of protons in bR.

Keywords

Permeability Magnesium Hydroxide Titration Carboxyl 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    W. Stoeckenius, R. H. Lozier, R. A. Bogomolni: Biophys. Biochim. Acta. 505, 215 (1979)CrossRefGoogle Scholar
  2. 2.
    A. Lewis, J. Spoonhower, R. A. Bogomolni, R. H. Lozier, W. Stoeckenius: Proc. Natl, Acad. Sci, USA. 71, 4462 (1974)ADSCrossRefGoogle Scholar
  3. 3.
    A. T. Quintanilha: FEBS Lett, 177, 8 (1980)CrossRefGoogle Scholar
  4. 4.
    S. L. Helgerson, M. K. Mathew, D. B. Bivin, P. K. Wolber, E. Heinz, W. Stoeckenius: Biophys, J. 48, 709 (1985)CrossRefGoogle Scholar
  5. 5.
    T. Kouyama, A. N. Kouyama, A. Ikegami: Biophys. J. (in press)Google Scholar
  6. 6.
    H. J. Weber, R. A. Bogomolni: Methods in Enzymology. 88, 379 (1982)CrossRefGoogle Scholar
  7. 7.
    A. E. Blaurock, W. Stoeckenius, D. Oesterhelt, & Scherphof G. L.: J. Cell Biol. 71, 1 (1976)CrossRefGoogle Scholar
  8. 8.
    Y. Mukohata, Y. Kaji: Arch. Biochem. Biophys. 208, 615 (1981)CrossRefGoogle Scholar
  9. 9.
    M. Eisenbach, H. Garty, E. P. Bakker, G. Klemperer, H. Rottenberg, S. R. Caplan: Biochemistry 17, 4691 (1978)CrossRefGoogle Scholar
  10. 10.
    T. Kouyama, A. N. Kouyama, A. Ikegami, M. K. Mathew, W. Stoeckenius: unpublished dataGoogle Scholar
  11. 11.
    A. Maeda, T. Ogura, T. Kitagawa: Biochemistry, 25, 2798 (1986)CrossRefGoogle Scholar
  12. 12.
    L. A. Drachev, A. D. Kaulen, V. P. Skulachev, V. V. Zorina: FEBS Lett. 209, 316 (1986)CrossRefGoogle Scholar
  13. 13.
    R. H. Lozier, W. Niederberger: Fed. Proc. 36, 1805 (1977)Google Scholar
  14. 14.
    K. Ohno, Y. Takeuchi, M. Yoshida: Photochem. Photobiol. 33, 573 (1981)CrossRefGoogle Scholar
  15. 15.
    Q. Li, R. Govindjee, T. G. Ebrey: Photochem. Photobiol. 44, 515 (1986)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • T. Kouyama
    • 1
  • A. N. Kouyama
    • 1
  • A. Ikegami
    • 1
  1. 1.The Institute of Physical and Chemical ResarchWako-shi, SaitamaJapan

Personalised recommendations