Cryoultramicrotomy for Autoradiography and Enzyme Cytochemistry

  • Peter M. Frederik


The introduction of cryoultramicrotomy as a new tool in histochemistry and cytochemistry has led to great expectations for autoradiography and enzyme cytochemistry, the classical methods in histochemistry and cytochemistry. It is an unpleasant task to summarize the present situation and to arrive at the conclusion that these promises are only partly fulfilled. For the autoradiography of diffusible substances, there seem to be only practical limitations (work and time), however, for enzyme cytochemistry, there are also theoretical limitations to the use of thin cryosections. Autoradiography and enzyme cytochemistry have thus hardly benefitted from the development of cryoultramicrotomy and this remains in sharp contrast to immunocytochemistry where the thin cryosection has found many applications and has become a sort of “(irnrmmo)-golden” standard (see also Boonstra et al., Chap. 11, this Vol.).


Photographic Emulsion Label Compound Chemical Fixation Electron Microscope Level Light Microscope Level 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • Peter M. Frederik
    • 1
  1. 1.Department of Pathology/EM UnitUniversity of MaastrichtMaastrichtThe Netherlands

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