Potato pp 427-440 | Cite as

Preservation of Potato Pollen

  • L. E. Towill
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 3)

Abstract

The development of useful procedures for pollen storage evolves from the need to utilize pollen at some later time after collection. Reasons for storage include (1) convenience in making genetic crosses between individuals one or both of which may be difficult to flower at any given time; (2) maintenance of pollen in a very uniform physiological state such that it is available for experiments over an extended time period, and (3) a means for storing and distributing germplasm of desirable species. Inherent within the latter is the use for international exchange of germplasm (Bajaj 1979). Although many potato diseases are not pollen transmitted, transmission of potato spindle tuber viroid (PSTV) by potato pollen (Singh 1970) may limit such exchanges unless assurances of the disease-free nature of the pollen are possible. Other minor uses for pollen storage may involve more specific goals such as preservation for allergenic activity studies, use in apiary culture and generation of desired monoploids and haploids through microspore or pollen culture. Only the latter minor use is presently of concern for potato.

Keywords

Hydrolysis Crystallization Sucrose Hydrated Boron 

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References

  1. Ashwood-Smith MJ, Grant E (1977) Genetic stability in cellular systems stored in the frozen state. In: The freezing of mammlian embryos. Ciba Found Symp 52, Elsevier/North Holland Biomedical Press, Amsterdam New York, pp 251–267Google Scholar
  2. Bajaj YPS (1979) Technology and prospects of cryopreservation of germplasm. Euphytica 28:267–285CrossRefGoogle Scholar
  3. Beamish K (1954) The effect of pollen aging on seed development in Solarium. Am Potato J 31:366 (Abstr)CrossRefGoogle Scholar
  4. Blomquist AW, Lauer FI (1962) A simplified technique for handling and storing potato pollen. Am Potato J 39:340–343CrossRefGoogle Scholar
  5. Brewbaker JL, Kwack BH (1983) The essential role of calcium ion in pollen germination and pollen tube growth. Am J Bot 50:859–865CrossRefGoogle Scholar
  6. Calcott PH, Gargett AM (1981) Mutagenicity of freezing and thawing. FEMS Microbiol Lett 10:151–155CrossRefGoogle Scholar
  7. DeMaine MJ (1977) A simple technique for the short-term storage of pollen in a dihaploid-inducing potato clone. J Agric Sci Cambridge 89:511–512CrossRefGoogle Scholar
  8. Hawkes JG (1979) Evolution and polyploidy in potato species. In: Hawkes JG, Lester RN, Skelding AD (eds) The biology and taxonomy of the solanaceae. Linnean Soc Symp Ser 7, Academic Press, London New York, pp 637–645Google Scholar
  9. Heslop-Harrison J, Heslop-Harrison Y (1970) Evaluation of pollen viability by enzymatically induced fluorescence: intracellular hydrolysis of fluorescein diacetate. Stain Technol 45:115–120Google Scholar
  10. Hodgkin T, Lyon GD (1979) Inhibition of Solanum pollen germination in vitro by the phytoalexin rishitin. Ann Bot (London) 44:253–255Google Scholar
  11. Howard HW (1958) The storage of potato pollen. Am Potato J 35:676–678CrossRefGoogle Scholar
  12. Janssen AWB, Hermsen JGTh (1976) Estimating pollen fertility in Solanum species and haploids. Euphytica 25:577–586CrossRefGoogle Scholar
  13. Johri BM, Vasil IK (1961) Physiology of pollen. Bot Rev 27:325–381CrossRefGoogle Scholar
  14. King JR (1955 a) Pollen and pollination studies on Irish potatoes. Am Potato J 32:7–14CrossRefGoogle Scholar
  15. King JR (1955b) Irish potato pollen storage. Am Potato J 32:460–466CrossRefGoogle Scholar
  16. King JR (1960) The peroxidase reaction as an indicator of pollen viability. Stain Technol 35:225–227Google Scholar
  17. King JR (1961) The freeze-drying of pollens. Econ Bot 15:91–98CrossRefGoogle Scholar
  18. King JR, Johnston TM (1958) Factors affecting Irish potato pollen germination in an artificial environment. Am Potato J 35:689–799CrossRefGoogle Scholar
  19. Linskens HF (1964) Pollen physiology. Annu Rev Plant Physiol 15:255–270CrossRefGoogle Scholar
  20. Mazur P (1966) Physical and chemical basis of injury in single-celled micro-organisms subjected to freezing and thawing. In: Meryman HT (ed) Cryobiology. Academic Press, London New York, pp 213–315Google Scholar
  21. Mendiburu AO, Peloquin SJ, Mok DWS (1974) Potato breeding with haploids and 2n gametes. In: Kasha KJ (ed) Haploids in higher plants: advances and potential. Guelph Univ Press, pp 249–258Google Scholar
  22. Meryman HT (1966) Review of biological freezing. In: Meryman HT (ed) Cryobiology. Academic Press, London New York, pp 1–114Google Scholar
  23. Mortenson LR, Peloquin SJ, Hougas RW (1964) Germination of Solanum pollen on artificial media. Am Potato J 41:322–328CrossRefGoogle Scholar
  24. Peloquin SJ, Hougas RW (1959) Decapitation and genetic markers as related to haploidy in Solanum tuberosum. Eur Potato J 2:176–183CrossRefGoogle Scholar
  25. Sanford JC, Hanneman RE Jr (1977) The common potato pollen collector and modified for bulk pollen collection. Am Potato J 54:573–574CrossRefGoogle Scholar
  26. Shivanna KR, Heslop-Harrison J (1981) Membrane state and pollen viability. Ann Bot (London) 47:759–779Google Scholar
  27. Simon PW, Peloquin SJ (1976) Pollen vigor as a function of mode of 2n gamete formation in potatoes. J Hered 67:204–208Google Scholar
  28. Singh R (1970) Seed transmission of potato spindle tuber virus in tomato and potato. Am Potato J 47:225–227CrossRefGoogle Scholar
  29. Stanley RG, Linskens HF (1974) Pollen biology, biochemistry, and management. Springer, Berlin Heidelberg New YorkGoogle Scholar
  30. Towill LE (1981) Liquid nitrogen preservation of pollen from tuber-bearing Solanum species. Hort Sci 16:177–179Google Scholar
  31. Visser T (1955) Germination and storage of pollen. Meded Landbouwhogesch Wageningen 55:1–68Google Scholar
  32. Weatherhead MA, Grout BWW, Henshaw GG (1978) Advantages of storage of potato pollen in liquid nitrogen. Potato Res 21:331–334CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • L. E. Towill
    • 1
  1. 1.Agricultural Research ServiceUS Department of AgricultureUSA

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