Potato pp 223-239 | Cite as

Markers for Identifying Somatic Hybrids in Potato

  • W. D. Benton
  • E. Shahin
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 3)


Parasexual transfer of genetic information through hybridization of somatic plant cells provides a powerful tool for increasing genetic variability in cultivated potato. It is no surprise that the origin of the existing variability in the cultivated potato has been often attributed solely to gene exchange between diploid and tetraploid cultivars of Solarium tuberosum (Hawkes 1962). The real challenge has been and probably continues to be, to introduce more genes from the wild species into both diploid and tetraploid populations of Solarium tuberosum. Since sterility barriers exist between some wild species and S. tuberosum, somatic hybridization may be the only means available for effecting gene transfer. However, the successful application of this technology has thus far been limited to a few examples (Butenko and Kuchko 1979; Barsby et al. 1984; Austin et al. 1985) because efficient methods to identify and select hybrid cells are lacking. Markers for identification or selection of somatic hybrids are required because yields of heterokaryons seldom exceed 1% of the protoplasts in a fusion mixture (Butenko and Kuchko 1979).


Selectable Marker Somatic Hybrid Hybrid Cell Protoplast Fusion Potato Leaf Roll Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • W. D. Benton
  • E. Shahin
    • 1
  1. 1.ARCO Plant Cell Research InstituteDublinUSA

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