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Potato pp 187–194Cite as

Improved Culture Techniques for Potato Protoplasts

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Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 3))

Abstract

The interest in regenerating potato plants through protoplast culture techniques has grown immensely during the last few years, probably due to the vast opportunities offered by this novel method for crop improvement. As a consequence, many different protocols have been published on the isolation and culture of protoplasts from this species (Butenko et al. 1977; Shepard 1980; Thomas 1981; Bokelmann and Roest 1983). Since none of these methods was suitable for the isolation and further culture of some potato varieties of interest to Swedish potato breeding, we had to make some modifications of previously described protocols, and these modifications are described in this chapter. We could increase the number of growing cell colonies from protoplasts by using activated charcoal in order to adsorb substances present in the culture, which otherwise had a deleterious effect on cell viability. Moreover, diluting the medium or lowering the auxin concentration had a promotive effect on plating efficiency.

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Author information

Authors and Affiliations

  1. Institute of Physiological Botany, University of Uppsala, Box 540, S-75121, Uppsala, Sweden

    T. Eriksson

Authors
  1. I. Carlberg
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  2. S. Karlsson
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  3. T. Eriksson
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Editor information

Editors and Affiliations

  1. A-137, New Friends Colony, New Delhi, 110065, India

    Y. P. S. Bajaj

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© 1987 Springer-Verlag Berlin Heidelberg

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Carlberg, I., Karlsson, S., Eriksson, T. (1987). Improved Culture Techniques for Potato Protoplasts. In: Bajaj, Y.P.S. (eds) Potato. Biotechnology in Agriculture and Forestry, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72773-3_13

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  • DOI: https://doi.org/10.1007/978-3-642-72773-3_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72775-7

  • Online ISBN: 978-3-642-72773-3

  • eBook Packages: Springer Book Archive

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