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Receptor Analysis: Data Processing in the Presence of Nonspecific Binding Sites

  • H. Moeller
  • Ch. Fusch
  • A. Kuch
  • N. Manolopoulos
  • G. Oettling
Conference paper

Abstract

In biochemical receptor analysis 2 parameters are of particular interest: the concentration of receptor binding sites and the dissociation constant of the receptor-ligand complex. For this purpose cell fractions or intact cells containing the receptor are incubated in vitro with radioactive ligand until equilibrium is achieved:
$${\text{L}} + {\text{R}} + \rightleftarrows {\text{RL}} + {\text{BL}}$$
(1)
L = free radioactive ligand, R = free receptor, B = free nonspecific binder, RL = radioactive receptor-ligand complex, BL = radioactive complex of ligand and non-specific binder.

Keywords

Receptor Binding Site Scatchard Plot Binding System Free Receptor Receptor Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Eisenthal R, Cornish-Bowden A (1974)The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem J 139: 715–720PubMedGoogle Scholar
  2. Oettling G (1987) Messung der freien und besetzten Androgenrezeptoren im Cytosol der ventralen Rattenprostata. Dissertation, TübingenGoogle Scholar
  3. Rosenthal HE (1967) A graphic method for the determination and presentation of binding parameters in a complex system. Anal Biochem 20: 525–532PubMedCrossRefGoogle Scholar
  4. Scatchard G (1949)The attractions of proteins for small molecules and ions. Ann NY Acad Sci 51: 660–672CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • H. Moeller
    • 1
  • Ch. Fusch
    • 1
  • A. Kuch
    • 1
  • N. Manolopoulos
    • 1
  • G. Oettling
    • 2
  1. 1.Children’s HospitalUniversity of TübingenTübingenGermany
  2. 2.Department of AnatomyUniversity of TübingenTübingenGermany

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