Receptor Analysis: Data Processing in the Presence of Nonspecific Binding Sites

  • H. Moeller
  • Ch. Fusch
  • A. Kuch
  • N. Manolopoulos
  • G. Oettling
Conference paper

Abstract

In biochemical receptor analysis 2 parameters are of particular interest: the concentration of receptor binding sites and the dissociation constant of the receptor-ligand complex. For this purpose cell fractions or intact cells containing the receptor are incubated in vitro with radioactive ligand until equilibrium is achieved:
$${\text{L}} + {\text{R}} + \rightleftarrows {\text{RL}} + {\text{BL}}$$
(1)
L = free radioactive ligand, R = free receptor, B = free nonspecific binder, RL = radioactive receptor-ligand complex, BL = radioactive complex of ligand and non-specific binder.

Keywords

Cytosol Rosen 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • H. Moeller
    • 1
  • Ch. Fusch
    • 1
  • A. Kuch
    • 1
  • N. Manolopoulos
    • 1
  • G. Oettling
    • 2
  1. 1.Children’s HospitalUniversity of TübingenTübingenGermany
  2. 2.Department of AnatomyUniversity of TübingenTübingenGermany

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