Tumor-Specific Antigen for Human Renal Cell Carcinoma: Ultrastructural Localization of the Antigen Using Immunoelectron Microscopy

  • T. Schärfe
  • St. Störkel
  • M. Yokoyama
  • R. Kaltwasser
  • G. H. Jacobi
  • R. Hohenfellner
Conference paper

Abstract

In clinical oncology, tumor markers are a valuable tool in therapy monitoring of tumor patients as well as for primary diagnosis. In renal cell carcinoma a number of tumor associated antigens were described which may also be expressed in normal kidney epithelium (Bander et al. 1983; Bander 1984; Moon et al. 1982; Oosterwijk et al. 1987a, b; Ueda 1981). Only occasionally are antigens described which do not react in normal kidney tissue. The here described antigen is restricted to well differentiated human renal cell carcinoma (RCC) and does not show any expression in the normal kidney or other human organs (Table 1). The antibodies produced by hybridoma-technology are highly specific for the antigen: they are IgG1 antibodies. The antibody-production was achieved according to the method of Köhler and Milstein (Köhler and Milstein 1975).

Keywords

Sucrose Glycerine Adenoma Lysine Stein 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • T. Schärfe
    • 1
  • St. Störkel
    • 2
  • M. Yokoyama
    • 1
  • R. Kaltwasser
    • 1
  • G. H. Jacobi
    • 1
  • R. Hohenfellner
    • 1
  1. 1.Department of Urology, Medical SchoolJohannes Gutenberg UniversityMainzGermany
  2. 2.Institute of PathologyJohannes Gutenberg UniversityMainzGermany

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