Hydration of Chlorophyll a

  • K. Rangsriwatananon
  • H. Kleeberg
Conference paper


The sorption isothermes and infrared spectra (4000–1000 cm-1) of Chlorophyll a (chl a) are compared.

Between 90 and 10 % relative humidity r.h. 1–2 H2O/chl a is present during desorption. The H2O spectra indicate the presence of two spectroscopically different H2O molecules containing free OH groups and H- bonds of intermediate strength. At low H2O contents (below 1 H2O chl a) H2O interactions are very different and strong H-bonds are formed. This H2O is desorbed only at elevated temperatures (80–100°C). During the subsequent H2O adsorption process water molecules form 2 moderately strong H-bonds with chl a. The changes in H2O interaction are reflected by the spectra of chl a in the C=O streching region as well.

The results are interpreted with respect to a cooperative influence of the magnesium-ion of chl a on the hydration.


Quartz Magnesium Ethyl Hydration Acetone 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ballschmiter K and Katz JJ (1968) Long Wavelength Forms of Chlorophyll. Nature 220: 1231CrossRefGoogle Scholar
  2. Ballschmiter K and Katz JJ (1972) Chlorophyll - Chlorophyll and Chlorophyll - Water Interactions in the solid state. BBA 256: 307PubMedCrossRefGoogle Scholar
  3. Behrens A and Luck WAP (1980). J Mol Struct 60: 333–342CrossRefGoogle Scholar
  4. Brown J (1977)- Photochem and Photobiol 26: 319CrossRefGoogle Scholar
  5. Cotton TM, Loach PA, Katz JJ and Ballschmiter K (1978). Photochem and Photobiol 27: 735CrossRefGoogle Scholar
  6. Fettermann LM, Galloway L, Winograd N and Fong FK (1977). J Amer Chem Soc 99: 653CrossRefGoogle Scholar
  7. Fong FK (1975). J Amer Chem Soc 97: 689OGoogle Scholar
  8. Fong FK and Koester VJ (1975). J Amer Chem Soc 97: 6888CrossRefGoogle Scholar
  9. Katz JJ and Ballschmiter K (1968). Angew Chem 80: 283CrossRefGoogle Scholar
  10. Katz JJ, Dougherty RC and Boucher LJ (1966) Infrared and NMR Spectroscopy of Chlorophyll. In: Vernon LP and Seely GR (eds) The Chlorophylls, Academic Press, New York and London, p. 187Google Scholar
  11. Kleeberg H. This volumeGoogle Scholar
  12. Kleeberg H, Heinje G and Luck WAP (1986). J Phys Chem 90: 4427CrossRefGoogle Scholar
  13. Koester VJ and Fong FK (1976). J Phys Chem 80: 2310CrossRefGoogle Scholar
  14. Kleeberg H, Klein D Luck WAP (1987). J Phys Chem in pressGoogle Scholar
  15. Schiöberg D and Luck WAP (1979)- J Chem Soc Farad Trans I 75: 762–773CrossRefGoogle Scholar
  16. Wolf W et al (1984). J Food Eng 3: 51CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • K. Rangsriwatananon
    • 1
  • H. Kleeberg
    • 1
  1. 1.Fachbereich Physikalische ChemieUniversität MarburgMarburgGermany

Personalised recommendations