Erythropoietin Production and Erythropoietin Receptors on Murine Erythroleukemic Cell Lines
In 1983 we described the first observation of a murine erythroleukemic cell line (IW32) producing high levels of erythropoietin (Epo). This cell line was isolated from a leukemic mouse infected by a replication-competent retrovirus isolated from the Friend virus complex. Erythropoietin characterization was achieved by biological, biochemical and immunological methods. It was demonstrated that Epo produced by IW32 was almost normal, slightly differing from native Epo at the glycosylation level.
Southern blot analysis of IW32 genomic DNA showed that one Epo gene was amplified and rearranged in this cell line. Ten other independent murine erythroleukemic cell lines were checked for Epo production either at the mRNA level or by testing biological activities of the cell supernatants. One other cell line (NN10) was found to produce rather high levels of Epo (0.5 U/ml). All the other cell lines were negative. In the NN10
KeywordsErythropoietin Production Erythroid Lineage Friend Virus Erythroleukemic Cell Line Renal Mesangial Cell
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