Studies on the Interaction between Murine Resident Bone Marrow Macrophages and Haematopoietic Cells
Murine resident bone marrow macrophages (RBMM), which form intimate associations with developing haematopoietic cells in situ, were isolated as intact clusters by collagenase digestion. After purification, RBMM were found to express high levels of a novel haemagglutinin for unopsonized sheep erythrocytes (SER). Sialic acid on erythrocytes was a minimal requirement for binding which was not accompanied by ingestion. High levels of SER could be induced on peritoneal macrophages by cultivation in homologous plasma. The induced haemagglutinin mediated the attachment of murine bone marrow cells which express an appropriate sialyated ligand for SER.
KeywordsSialic Acid Mouse Bone Marrow Sheep Erythrocyte Haematopoietic Cell Murine Bone Marrow Cell
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