Summary
Murine resident bone marrow macrophages (RBMM), which form intimate associations with developing haematopoietic cells in situ, were isolated as intact clusters by collagenase digestion. After purification, RBMM were found to express high levels of a novel haemagglutinin for unopsonized sheep erythrocytes (SER). Sialic acid on erythrocytes was a minimal requirement for binding which was not accompanied by ingestion. High levels of SER could be induced on peritoneal macrophages by cultivation in homologous plasma. The induced haemagglutinin mediated the attachment of murine bone marrow cells which express an appropriate sialyated ligand for SER.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Dexter TM (1982). Stromal cell-associated haempoiesis. J Cell Physiol Suppl 1: 87–94.
Lichtman MA (1981). The ultrastructure of the hemopoietic environemnt of the marrow: A review. Exp Hematol 9: 391–410.
Weiss L and Chen LT (1975). The organisation of hematopoietic cords and vascular sinuses in the bone marrow. Blood Cells 1: 617–638.
Allen TD and Dexter TM (1984). The essentials of the haemopoietic microenvironment. Exp Hematol 12: 517–521.
Bessis M (1958). L’ilot erythroblastique.Inite fonctionelle de la moelle osseuse. Rev Hemat 13: 8–11.
Besis M, Mize C and Prenant M (1978). Erythropoiesis: comparison of in vivo and in vitro amplification. Blood Cells 4: 153–174.
Prenant M (1980). Maturation of erythroblastic bone marrow cells in mammals. Biol Cellulaire 38: 9–12.
Allen TD and Dexter TM (1982). Ultrastructural aspects of erythropoietic differentiation in long-term bone marrow culture. Differentiation 21: 86–94.
Hume DA, Loutit JF and Gordon S (1984). The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80. Macrophages of bone and associated connective tissue. J Cell Sci 66: 189–194.
Crocker PR and Gordon S (1985), Isolation and characterisation of resident stromal macrophages and hematopoietic cell clusters from mouse bone marrow. J Exp Med 162: 993–1014.
Crocker PR and Gordon S (1986). Properties and distribution of a lectin-like haemagglutinin differentially expressed by murine stromal tissue macrophages. J Exp Med. In Press.
Westen H and Bainton DF (1979). Association of alkaline phosphatase postive reticulum cells in bone marrow with granulocytic precursors. J Exp Med 150: 919–937.
Allen TD, Simmons PJ and Dexter TM (1984). Commentary. Blood Cells 10: 467–471.
Metcalf D (1984). The haematopoietic colony stimulating factors. Elsevier Science Publishing Co., Amsterdam.
Hume DA and Gordon S (1983). The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80. Identification of resident macrophages in renal medullary and cortical interstitium and the justaglomerular complex.J Exp Med 157: 1704–1709.
Bessis M (1965). Cellular mechanisms for the destruction of erythrocytes. Scand J Haemat Series Haematologica 2: 59–68.
Springer TA and Anderson DC (1986). The importance of the Mac-1, LFA-1 glycoproetin family in monocyte and granulocyte adherence, chemotaxis, and migration into inflammatory sites: insights from an experiment of nature. In: Biochemistry of Macrophages. Ciba Fdn Symp 118: 102–126.
Hogg N, Selvendran Y, Dougherty G and Allen C(1986).Macrophage antigens and the effect of a macrophage activating factor, interferon-. In: Biochemistry of Macrophages. Ciba Fdn Symp 118: 68–80.
Moore MAS (1978). Regulatory role of the macrophage in haemopoiesis. In: Lord BI, Potten CS, Cole RJ (eds), Stem Cells and Tissue Homeostasis. Cambridge, Cambridge University Press, pp 187–202.
Cline MJ and Golde DW (1979). Cellular interactions in haematopoiesis. Nature 277: 177–181.
Bagby GC, McCall E and Layman DL (1983). Regulation of colony stimulating activity production. Interactions with fibroblasts, mononuclear phagocytes and lactoferrin. J Clin Invest 71: 340–346.
Kleinman HK, Martin GR and Fishman PH (1979). Ganglioside inhibition of fibronectin-mediated cell adhesion to collagen. Proc Natl Acad Sci USA 76: 3367–3371.
Kennedy DW, Rohrbach DH, Martin GR, Momoi T and Yamada KM (1983). The adhesive glycoproetin laminin is an agglutinin. J Cell Physiol 114: 257–262.
Brown EJ (1986). The role of extracellular matrix proteins in the control og phagocytosis. J Leukocyte Biol 39: 579–591.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Crocker, P.R., Gordon, S. (1987). Studies on the Interaction between Murine Resident Bone Marrow Macrophages and Haematopoietic Cells. In: Rich, I.N. (eds) Molecular and Cellular Aspects of Erythropoietin and Erythropoiesis. NATO ASI Series, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72652-1_20
Download citation
DOI: https://doi.org/10.1007/978-3-642-72652-1_20
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-72654-5
Online ISBN: 978-3-642-72652-1
eBook Packages: Springer Book Archive