Studies on the Interaction between Murine Resident Bone Marrow Macrophages and Haematopoietic Cells
Murine resident bone marrow macrophages (RBMM), which form intimate associations with developing haematopoietic cells in situ, were isolated as intact clusters by collagenase digestion. After purification, RBMM were found to express high levels of a novel haemagglutinin for unopsonized sheep erythrocytes (SER). Sialic acid on erythrocytes was a minimal requirement for binding which was not accompanied by ingestion. High levels of SER could be induced on peritoneal macrophages by cultivation in homologous plasma. The induced haemagglutinin mediated the attachment of murine bone marrow cells which express an appropriate sialyated ligand for SER.
KeywordsMigration Magnesium Sedimentation Interferon Trypsin
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- 3.Weiss L and Chen LT (1975). The organisation of hematopoietic cords and vascular sinuses in the bone marrow. Blood Cells 1: 617–638.Google Scholar
- 6.Besis M, Mize C and Prenant M (1978). Erythropoiesis: comparison of in vivo and in vitro amplification. Blood Cells 4: 153–174.Google Scholar
- 7.Prenant M (1980). Maturation of erythroblastic bone marrow cells in mammals. Biol Cellulaire 38: 9–12.Google Scholar
- 11.Crocker PR and Gordon S (1986). Properties and distribution of a lectin-like haemagglutinin differentially expressed by murine stromal tissue macrophages. J Exp Med. In Press.Google Scholar
- 14.Metcalf D (1984). The haematopoietic colony stimulating factors. Elsevier Science Publishing Co., Amsterdam.Google Scholar
- 15.Hume DA and Gordon S (1983). The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80. Identification of resident macrophages in renal medullary and cortical interstitium and the justaglomerular complex.J Exp Med 157: 1704–1709.PubMedCrossRefGoogle Scholar
- 16.Bessis M (1965). Cellular mechanisms for the destruction of erythrocytes. Scand J Haemat Series Haematologica 2: 59–68.Google Scholar
- 17.Springer TA and Anderson DC (1986). The importance of the Mac-1, LFA-1 glycoproetin family in monocyte and granulocyte adherence, chemotaxis, and migration into inflammatory sites: insights from an experiment of nature. In: Biochemistry of Macrophages. Ciba Fdn Symp 118: 102–126.CrossRefGoogle Scholar
- 19.Moore MAS (1978). Regulatory role of the macrophage in haemopoiesis. In: Lord BI, Potten CS, Cole RJ (eds), Stem Cells and Tissue Homeostasis. Cambridge, Cambridge University Press, pp 187–202.Google Scholar