Growth of the HTLV-III Strain of Human Immunodeficiency Virus in Different Cell Types

  • E. M. Fenyö
  • B. Åsjö
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 31)

Abstract

The major immunological abnormality in the acquired immunodeficiency syndrome (AIDS) appears to be a quantitative defect in the T4 antigen-positive helper/inducer T-cell subset. AIDS is etiologically linked to a retrovirus, designated human immunodeficiency virus (HIV), that has been shown to selectively infect T4 antigen-positive lymphoid cells in vitro [1]. Since virus replication in vitro is associated with a pronounced cytopathic effect, it has been suggested that

Table 1. Growth of the HTLV-IIIB isolate in different cell types

Cell typea

T4b antigen positive cells (%)

Virusc dose (cpm × 103/106 cells)

No. of Ex periments

Weeks after infection

              

Continuously producing line established

Viras detected by co-cultivation with U937-16

                    
    

1

  

2

  

3

  

4

  

9

                        
    

RT

IFd (%+)

CPEe

RT

IF (% +)

CPE

RT

IF

CPE

RT

IF

CPE

RT

IF

CPE

                      

PBMC

 

32

15

26

 

+

72

 

+

 

(% +)

  

(%+)

  

(% +)

                       

Monocytes

 

180

3

68.4

 

31

 

2.5

 

0.5

 

                         

T-cell lines

         

Gradual cell death

                              

H9

 

75

10

8

10

+

35

64

+

3.0

 

Gradual cell death

                           
  

2.5

2

 

 

64

70

+

110

80

++

   

yes

                     

HUT-78

 

60

1

5.8

 

+

143

 

++

 

64

 

++

                         
  

12

1

2.8

 

+

65

 

++

135

 

+

161

 

   

yes

                     

Karpas45

 

70

2

4.8

7

+

293

83

+++

134

 

+

49

 

   

yes

                     

Molt-3

 

50

2

104

 

+++

      

no

                     

Monocytoid cell line and derived clones

         

 

72

5

+

   

yes

                     

U937 parental

<10

150

1

  

  

 

 

79

90

yes

                     

U937 clone 4

<10

1800

1

6.5

 

3

 

12

 

19

 

170

 

yes

                     

U937 clone 1

50–60

350

1

1.3

 

1.7

 

+

35

 

++

65

70

   

yes

                     

U937 clone 16

>95

100

15

80

95

+++

            

no

                     
  

25

2

15

 

++

  

+++

  

+++

                            
  

2.5

2

0.4

 

5.8

 

++

20

no

+

                    

Malignant glioma cell lines

         

no

                    

138

0

100

2

2

no

                    

373

0

100

2

                               

489

0

100

2

                               

a Peripheral blood mononuclear cells were grown in RPMI medium supplemented with 10% fetal calf serum (FCS), 10% T-cell growth factor (Cellular Products), 45 IU of sheep anti-human α-interferon serum, and 2 μg/ml polybrene (PB). Monocytes, T-cell lines, and monocytoid cell lines were grown in RPMI medium with 10% FCS and 2 μg/ml PB. Malignant gliomas were grown in Eagles MEM with 10% FCS and PB.

b Indirect membrane immuniofluorescence with the Fab fragment of an anti-T4 monoclonal antibody (received from Dr. Ellis Reinherz) and fluorescein isothiocyanate (FITC)-labeled rabbit anti-mouse IgG (Dakopatts, Glostrup, Denmark).

c Estimated by reverse transcriptase activity (RT) [5].

d Immunofluorescence on methanol-fixed cells with monoclonal antibodies to HTLV-III p24 and p15.

e Cytopathic effect: syncytia formation and cell death; +, <5% of cells, ++, 5%–50%, + + +,

>50% of cells show CPE.

immunodeficiency in vivo is a result of the virus killing the T4 cells. To gain further insight into the virus-cell interactions we studied the replication of the HTLV-IIIB strain of HIV in different cell types.

Keywords

Lymphoma Leukemia Producer Line Fluorescein FITC 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • E. M. Fenyö
    • 1
  • B. Åsjö
    • 1
  1. 1.Department of VirologyKarolinska InstitutetStockholmSweden

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