Complete Nucleotide Sequences of Functional Clones of the Virus Associated with the Acquired Immunodeficiency Syndrome, HTLV-III/LAV
There is considerable evidence that the human T-lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/ LAV) is the etiological agent in the acquired immunodeficiency syndrome (AIDS) and AIDS-related syndromes . The most convincing line of evidence is the recapitulation with in vitro infection of the major manifestation of the disease, depletion of T4 cells . Definition of the viral determinants of this lymphocytopathic activity is critical to understanding the pathogenesis of AIDS. With this goal in mind we have established an in vitro model which will facilitate this analysis. In this system, plasmid clones with the full HTLV-III/LAV proviral sequence are transfected into normal human umbilical cord blood mononuclear cell cultures. This results in production of virus particles with a morphology typical of HTLV-III/LAV and in death of the cell culture (Fig. 1).
KeywordsAcquire Immunodeficiency Syndrome Complete Nucleotide Sequence Proviral Sequence Hybridization Western Blot Viral Probe
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