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Complete Nucleotide Sequences of Functional Clones of the Virus Associated with the Acquired Immunodeficiency Syndrome, HTLV-III/LAV

  • L. Ratner
  • A. Fisher
  • L. L. Jagodzinski
  • R.-S. Liou
  • H. Mitsuya
  • R. C. Gallo
  • F. Wong-Staal
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 31)

Abstract

There is considerable evidence that the human T-lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/ LAV) is the etiological agent in the acquired immunodeficiency syndrome (AIDS) and AIDS-related syndromes [1]. The most convincing line of evidence is the recapitulation with in vitro infection of the major manifestation of the disease, depletion of T4 cells [2]. Definition of the viral determinants of this lymphocytopathic activity is critical to understanding the pathogenesis of AIDS. With this goal in mind we have established an in vitro model which will facilitate this analysis. In this system, plasmid clones with the full HTLV-III/LAV proviral sequence are transfected into normal human umbilical cord blood mononuclear cell cultures. This results in production of virus particles with a morphology typical of HTLV-III/LAV and in death of the cell culture (Fig. 1).

Keywords

Acquire Immunodeficiency Syndrome Complete Nucleotide Sequence Proviral Sequence Hybridization Western Blot Viral Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Gallo RC, Wong-Staal F (1985) A human T-lymphotropic retrovirus (HTLV-III) as the cause of the acquired immunodeficiency syndrome. Ann Int Med 103:679–689PubMedGoogle Scholar
  2. 2.
    Popovic M, Sarngadharan MG, Read E, Gallo RC (1984) Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224:497–500PubMedCrossRefGoogle Scholar
  3. 3.
    Ratner L, Fisher A, Jagodzinski LJ, Mitsuya H, Liou R-S, Gallo RC, Wong-Staal F (1987) Complete nucleotide sequences of functional clones of the virus associated with the acquired immunodeficiency syndrome, HTLV-III/LAV. AIDS Res & Hum Retrov 3Google Scholar
  4. 4.
    Ratner L, Haseltine W, Patarca R, Livak KJ, Starcich B, Josephs SF, Doran ER, Rafalski JA, Whitehorn EA, Baumeister K, Ivanoff L, Petteway SR, Pearson ML, Lautenberger JA, Papas TS, Ghrayeb J, Chang NT, Gallo RC, Wong-Staal F (1985) Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature 313:277–284PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • L. Ratner
    • 1
  • A. Fisher
    • 2
  • L. L. Jagodzinski
    • 3
  • R.-S. Liou
    • 3
  • H. Mitsuya
    • 4
  • R. C. Gallo
    • 2
  • F. Wong-Staal
    • 2
  1. 1.Division of Hematology/OncologyWashington UniversitySt. LouisUSA
  2. 2.Laboratory of Tumor Cell BiologyNational Cancer InstituteBethesdaUSA
  3. 3.Biotech Research Laboratories, Inc.RockvilleUSA
  4. 4.The Clinical Oncology ProgramNational Cancer InstituteBethesdaUSA

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