Abstract
An emerging, important characteristic of many leukemic cell types is their altered dependence on and/or response to hematopoietic growth factors [6, 17]. In mammals, many of these growth-regulatory proteins have been purified and the respective genes molecularly cloned [16, 26], but the mechanism by which they regulate growth and differentiation of normal hematopoietic precursors is still poorly understood. This is due partly to the fact that such hematopoietic precursors do not self-renew in vitro and constitute only a minor fraction of bone marrow cells, precluding their purification in large numbers [25]. One particularly successful approach to circumventing this problem was the use of avian retroviral oncogenes that transform hematopoietic precursors [10].
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beug H, Palmieri S, Freudenstein C, Zentgraf H, Graf T (1982) Hormone-dependent terminal differentiation in vitro of chicken erythroleukemia cells transformed by ts mutants of avian erythroblastosis virus. Cell 28:907–919
Beug H, Hayman MJ, Graf T, Benedict SH, Wallbank AM, Vogt PK (1985) S13, a rapidly oncogenic replication-defective avian retro-virus. Virology 145:141–153
Beug H, Kahn P, Doederlein G, Hayman MJ, Graf T (1985) Characterization of hematopoietic cells transformed in vitro by AEV-H, an erbB-containing avian erythroblastosis virus. In: Neth R, Gallo R, Greaves M, Janka K (eds) Modern trends in human leukemia, VI. Springer, Berlin Heidelberg New York Tokyo, pp 290–297
Beug H, Kahn P, Vennstroem B, Hayman MJ, Graf T (1985) How do retroviral oncogenes induce transformation in avian erythroid cells? Proc R Soc London [Biol] 226:121–126
Coll J, Ingram VM (1981) Identification of ovotransferrin as a heme-, colony- and burst-stimulating factor in chick erythroid cell cultures. Exp Cell Res 131:173–184
Cook WD, Metcalf D, Nicola N, Burgess A, Walker F (1985) Malignant transformation of a growth factor-dependent myeloid cell line by Abelson virus without evidence of an autocrine mechanism. Cell 41:677–683
Frykberg L, Palmieri S, Beug H, Graf T, Hayman MJ, Vennstroem B (1983) Transforming capacities of avian erythroblastosis virus mutants deleted in the erbA or erbB oncogenes. Cell 32:227–238
Gazzolo L, Moscovici C, Moscovici MG, Samarut J (1979) Response of hemopoietic cells to avian acute leukemia viruses: effects on the differentiation of the target cells. Cell 16:627–638
Graf T, Ade N, Beug H (1978) Temperature-sensitive mutant of avian erythroblastosis virus suggests a block of differentiation as mechanism of leukaemogenesis. Nature 257:496–501
Graf T, Beug H (1978) Avian leukemia viruses: interaction with their target cells in vivo and in vitro. BBA Revs Cancer 516:269–299
Graf T, von Kirchbach A, Beug H (1981) Characterization of the hematopoietic target cells of AEV, MC29 and AMV avian leukemia viruses. Exp Cell Res 131:331–343
Kahn P, Adkins B, Beug H, Graf T (1984) Src- and fps-containing avian sarcoma viruses transform chicken erythroid cells. Proc Natl Acad Sci USA 81:7122–7126
Kahn P, Frykberg L, Brady C, Stanley IJ, Beug H, Vennstroem B, Graf T (1986) V-erbA cooperates with sarcoma oncogenes in leukemic cell transformation. Cell 45:349–356
King GL, Kahn CR (1981) Non-parallel evolution of metabolic and growth-promoting function of insulin. Nature 292:644–646
Leutz A, Beug H, Graf T (1984) Purification and characterization of cMGF, a novel chicken myelomonocytic growth factor. EMBO J 3:3191–3197
Nicola NA, Metcalf D (1984) Binding of the differentiation-inducer, granulocyte-colony-stimulating factor to responsive but not unresponsive leukemia cell lines. Proc Natl Acad Sci USA 81:3765–3769
Pierce JH, DiGiore PP, Aaronson SA, Potter M, Pumphrey J, Scott A, Ihle JN (1985) Neoplastic transformation of mast cells by Abelson-MuLV: abrogation of IL-3 dependence by a nonautocrine mechanism. Cell 41:685–693
Petrides PE, Bohler P (1980) The mitogenic activity of insulin: an intrinsic property of the molecule. Biochem Biophys Res Commun 95:1138–1141
Radke K, Beug H, Kornfeld S, Graf T (1982) Transformation of both erythroid and myeloid cells by E26, an avian leukemia virus that contains the myb gene. Cell 31:643–653
Samarut J (1978) Isolation of an erythropoietic stimulating factor from the serum of anemic chicks. Exp Cell Res 115:123–126
Samarut J, Gazzolo L (1982) Target cells infected by avian erythroblastosis virus differentiate and become transformed. Cell 28:921–929
Spivak JL, Small D, Shaper JH, Hollenberg MD (1978) Use of immobilized lectin and other ligands for the partial purification of erythropoietin. Blood 52:1178–1186
Spivak JL, Small D, Hollenberg MD (1977) Erythropoietin: isolation by affinity chromatography with lectin-agarose derivates. Proc Natl Acad Sci USA 74:4633–4635
Schmidt JA, Marshall J, Hayman MJ, Ponka P, Beug H (1986) Control of erythroid differentiation: possible role of the transferrin cycle. Cell 46:41–51
van Zant G, Goldwasser E (1984) Erythropoietin and its target cells. In: Guroff G (ed) Growth and maturation factors, vol 2. Wiley, New York
Walker F, Burgess AW (1985) Specific binding of radioiodinated granulocyte-macrophage colony stimulating factor to hematopoietic cells. EMBO J 4:933–939
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Kowenz, E., Leutz, A., Döderlein, G., Graf, T., Beug, H. (1987). ts-Oncogene-Transformed Erythroleukemic Cells: A Novel Test System for Purifying and Characterizing Avian Erythroid Growth Factors. In: Neth, R., Gallo, R.C., Greaves, M.F., Kabisch, H. (eds) Modern Trends in Human Leukemia VII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72624-8_44
Download citation
DOI: https://doi.org/10.1007/978-3-642-72624-8_44
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-17754-8
Online ISBN: 978-3-642-72624-8
eBook Packages: Springer Book Archive