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ts-Oncogene-Transformed Erythroleukemic Cells: A Novel Test System for Purifying and Characterizing Avian Erythroid Growth Factors

  • E. Kowenz
  • A. Leutz
  • G. Döderlein
  • T. Graf
  • H. Beug
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 31)

Abstract

An emerging, important characteristic of many leukemic cell types is their altered dependence on and/or response to hematopoietic growth factors [6, 17]. In mammals, many of these growth-regulatory proteins have been purified and the respective genes molecularly cloned [16, 26], but the mechanism by which they regulate growth and differentiation of normal hematopoietic precursors is still poorly understood. This is due partly to the fact that such hematopoietic precursors do not self-renew in vitro and constitute only a minor fraction of bone marrow cells, precluding their purification in large numbers [25]. One particularly successful approach to circumventing this problem was the use of avian retroviral oncogenes that transform hematopoietic precursors [10].

Keywords

Erythroid Cell Erythroid Differentiation Hematopoietic Precursor Chicken Serum Tyrosine Kinase Oncogene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • E. Kowenz
    • 1
  • A. Leutz
    • 1
  • G. Döderlein
    • 1
  • T. Graf
    • 1
  • H. Beug
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergGermany

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