ts-Oncogene-Transformed Erythroleukemic Cells: A Novel Test System for Purifying and Characterizing Avian Erythroid Growth Factors
An emerging, important characteristic of many leukemic cell types is their altered dependence on and/or response to hematopoietic growth factors [6, 17]. In mammals, many of these growth-regulatory proteins have been purified and the respective genes molecularly cloned [16, 26], but the mechanism by which they regulate growth and differentiation of normal hematopoietic precursors is still poorly understood. This is due partly to the fact that such hematopoietic precursors do not self-renew in vitro and constitute only a minor fraction of bone marrow cells, precluding their purification in large numbers . One particularly successful approach to circumventing this problem was the use of avian retroviral oncogenes that transform hematopoietic precursors .
KeywordsErythroid Cell Erythroid Differentiation Hematopoietic Precursor Chicken Serum Tyrosine Kinase Oncogene
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