Human Follicular Lymphomas: Identification of a Second t(14;18) Breakpoint Cluster Region
Most follicular lymphomas, which comprise nearly two-thirds of the non-Hodgkin’s lymphomas occurring in U.S. adults, have been shown by cytogenetic analyses to contain a t(14;18) translocation [1, 2]. This breakpoint on chromosome 14 has been localized to band 14q32, the site of the immunoglobulin heavy chain genes. It was thus possible to use human Ig gene fragments to clone out a breakpoint DNA fragment (pFL-1) from tissue biopsy specimens of these lymphomas and from cell lines containing this translocation. When used as a hybridization probe, rearranged pFL-1 containing DNA was detected in approximately 60% of follicular lymphomas. Thus, a significant percentage of follicular lymphomas failed to show a breakpoint on chromosome 18 within 15–20 kb on either side of the breakpoint region. We describe here the cloning of a chromosome 18 DNA fragment (pFL-2) that detects t(14;18) rearranged DNA in most of these negative follicular lymphomas.
KeywordsFollicular Lymphoma Cluster Region Hybrid Cell Line Genomic Southern Blot Immunoglobulin Heavy Chain Gene
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