Abstract
The permissive or inductive environment provided by bone marrow stromal cells in long-term cultures allows the persistent proliferation and differentiation of haemopoietic stem and progenitor cells [1]. Cells harvested from cultures of murine bone marrow can reconstitute the haemopoietic system when transplanted into lethally irradiated mice [2]. If bone marrow heavily infiltrated with leukaemic cells is cultured using similar conditions, the leukaemic blast cells become undetectable within 1 week, and the cultured marrow can then be used to rescue lethally irradiated mice [3]. In cultures of bone marrow cells from untreated patients with chronic granulocytic leukaemia (CML), the Philadelphia chromosome decreased, after being present in over 95% of mitoses, to almost undetectable levels. In the same cultures, Ph-negative cells became the predominant mitotic population [4].
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References
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© 1987 Springer-Verlag Berlin Heidelberg
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Testa, N.G., Coutinho, L., Chang, J., Morgenstern, G., Scarffe, J.H., Dexter, T.M. (1987). The Use of Cultured Bone Marrow Cells for Autologous Transplantation in Patients with Acute Myeloblastic Leukemia. In: Neth, R., Gallo, R.C., Greaves, M.F., Kabisch, H. (eds) Modern Trends in Human Leukemia VII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72624-8_16
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DOI: https://doi.org/10.1007/978-3-642-72624-8_16
Publisher Name: Springer, Berlin, Heidelberg
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