The Use of Cultured Bone Marrow Cells for Autologous Transplantation in Patients with Acute Myeloblastic Leukemia
The permissive or inductive environment provided by bone marrow stromal cells in long-term cultures allows the persistent proliferation and differentiation of haemopoietic stem and progenitor cells . Cells harvested from cultures of murine bone marrow can reconstitute the haemopoietic system when transplanted into lethally irradiated mice . If bone marrow heavily infiltrated with leukaemic cells is cultured using similar conditions, the leukaemic blast cells become undetectable within 1 week, and the cultured marrow can then be used to rescue lethally irradiated mice . In cultures of bone marrow cells from untreated patients with chronic granulocytic leukaemia (CML), the Philadelphia chromosome decreased, after being present in over 95% of mitoses, to almost undetectable levels. In the same cultures, Ph-negative cells became the predominant mitotic population .
KeywordsLeukemia Radium Oncol Interferon Thrombocytopenia
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