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The Use of Cultured Bone Marrow Cells for Autologous Transplantation in Patients with Acute Myeloblastic Leukemia

  • N. G. Testa
  • L. Coutinho
  • J. Chang
  • G. Morgenstern
  • J. H. Scarffe
  • T. M. Dexter
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 31)

Abstract

The permissive or inductive environment provided by bone marrow stromal cells in long-term cultures allows the persistent proliferation and differentiation of haemopoietic stem and progenitor cells [1]. Cells harvested from cultures of murine bone marrow can reconstitute the haemopoietic system when transplanted into lethally irradiated mice [2]. If bone marrow heavily infiltrated with leukaemic cells is cultured using similar conditions, the leukaemic blast cells become undetectable within 1 week, and the cultured marrow can then be used to rescue lethally irradiated mice [3]. In cultures of bone marrow cells from untreated patients with chronic granulocytic leukaemia (CML), the Philadelphia chromosome decreased, after being present in over 95% of mitoses, to almost undetectable levels. In the same cultures, Ph-negative cells became the predominant mitotic population [4].

Keywords

Acute Myeloblastic Leukaemia Leukaemic Cell Murine Bone Marrow Culture Bone Marrow Cell Chronic Granulocytic Leukaemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • N. G. Testa
    • 1
  • L. Coutinho
    • 1
  • J. Chang
    • 2
  • G. Morgenstern
    • 2
  • J. H. Scarffe
    • 3
  • T. M. Dexter
    • 1
  1. 1.Department of Experimental Haematology, Paterson LaboratoriesChristie Hospital and Holt Radium InstituteManchesterEngland
  2. 2.Department of HaematologyChristie Hospital and Holt Radium InstituteManchesterEngland
  3. 3.Department of Medical OncologyChristie Hospital and Holt Radium InstituteManchesterEngland

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