Na+/K+-pump mutant with a slow charge translocating step
During its transport cycle the Na+/K+-pump transport 3 Na+ ions out of and to K+ ions into the cells. The translocation of the Na+ ions is associated with a net movement of charge across the membrane. Charge translocation by the Na+/K+-pump can be studied as ouabain-sensitive transient current after fast voltage perturbation under Na/Na exchange conditions (5,6). The translocation of charges has been associated with the release of Na+ ions across an external high field access channel (2,7). The structure responsible for Na+ ion translocation is not known.
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