Arginine Appears not to be Located in the Active Site of Na+/K+-ATPase
It was found that modification of Na+/K+-ATPase by arginine specific reagents produced inhibition of the enzyme activity (1,2,4). ATP (1,2,4) and ADP (1,2) protected Na+/K+-ATPase against the inactivation. These data supported the idea that an arginine residue is located in nucleotide binding site of Na+/K+ATPase. We have compared inhibition of Na+K+ATPase from duck salt gland by two arginine specific reagents 2,3-butanedione and phenylglyoxal in the presence of three essential ligands — Na+, K+ and ATP.
KeywordsATPase Activity Slow Phase Borate Buffer Arginine Residue Nucleotide Binding Site
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- 4.Pedemonte CH, Kaplan JH (1988) Modification of arginine residues in the Na +,K+-ATPase. Biophys J 53: 222a.Google Scholar