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Cloning of the Dog Na+/K+-ATPase α1-Subunit

  • Z. J. Xie
  • H. Li
  • Q. Liu
  • Y. Wang
  • A. Askari
  • R. W. Mercer

Abstract

Many of the kinetic, proteolytic and structural studies on the Na+/K+-ATPase have been conducted on the enzyme isolated from the dog kidney. While the cDNA for the dog βlsubunit is available (1), the cDNA coding for the a-subunit has not been isolated. The availability of the dog a-subunit cDNA will make it possible to compare previous studies with results obtained from the expression of the dog kidney subunit. The purpose of this study was to isolate the cDNA coding for the a-subunit from the dog kidney.

Keywords

Sickle Cell Anemia Subunit cDNA Dilute Reaction Mixture Couple Reticulocyte Lysate System Sheep cDNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1994

Authors and Affiliations

  • Z. J. Xie
    • 1
    • 2
  • H. Li
    • 1
    • 2
  • Q. Liu
    • 1
    • 2
  • Y. Wang
    • 1
    • 2
  • A. Askari
    • 1
    • 2
  • R. W. Mercer
    • 1
    • 2
  1. 1.Department of PharmacologyMedical College of OhioToledoUSA
  2. 2.Department of Cell Biology and PhysiologyWashington University School of MedicineSt. LouisUSA

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