Identification of epitopes recognized by Na+/K+-ATPase-directed antibodies

  • H. Homareda
  • Y. Nagano
  • H. Matsui


It would be expected that the surface of the Na+/K+-ATPase molecule is responsible for the antigenic properties of the enzyme than the interior. Therefore, we examined the surface of the intact Na+/K+-ATPase molecule to identify the epitopes recognized by polyclonal antibodies directed against it. The data obtained suggested that K508*, a putative ATP binding site, is located on the surface of the enzyme molecule, whereas other putative ATP binding sites, and the phosphorylation site at D376*, are located in the interior of the molecule.


ATPase Activity Phosphorylation Site Trypsin Digestion Antigenic Property ATPase Molecule 
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Copyright information

© Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1994

Authors and Affiliations

  • H. Homareda
    • 1
  • Y. Nagano
    • 1
  • H. Matsui
    • 1
  1. 1.Department of BiochemistryKyorin University School of MedicineTokyo 181Japan

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