Identification of epitopes recognized by Na+/K+-ATPase-directed antibodies
It would be expected that the surface of the Na+/K+-ATPase molecule is responsible for the antigenic properties of the enzyme than the interior. Therefore, we examined the surface of the intact Na+/K+-ATPase molecule to identify the epitopes recognized by polyclonal antibodies directed against it. The data obtained suggested that K508*, a putative ATP binding site, is located on the surface of the enzyme molecule, whereas other putative ATP binding sites, and the phosphorylation site at D376*, are located in the interior of the molecule.
KeywordsEDTA Electrophoresis Trypsin Stein Coomassie
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