Functional Analysis of the Disulfide Bonds of Na+/K+-ATPase β-subunit by Site-directed Mutagenesis
The ion transporting Na+/K+-ATPase of animal plasma membranes consists of the catalytic α- and the glycosylated β-subunit All the functional roles in the catalytic activity of the enzyme so far known belong to the α-subunit, whereas those of the β-subunit remain still unknown. However, the β-subunit has been revealed to play important roles in the biogenesis of the enzyme. It acts as a stabilizer of the nascent α-subunit within the endoplasmic reticulum [9, 10] and takes part in targeting the resulting αβ -complex to the plasma membrane [2,12].
KeywordsCysteine Electrophoresis Serine Trypsin Polyacrylamide
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