Abstract
Identification of domains involved in assembly of the Na,K-ATPase α- and β- subunits is a major focus of our lab. These studies can lead to an understanding of the mechanisms controlling subunit assembly and may provide information about the membrane topology of the α-subunit and folding properties of the β-subunit extracellular domains. Our approach has taken advantage of the unique properties of several monoclonal antibodies that recognize the chicken β1 -subunit. Expression of chimeric subunits and/or subunits lacking portions of the polypeptide chains, followed by immune precipitations with these monoclonal antibodies, has allowed us to identify minimal regions within both the α- and β-subunit amino acid sequences that are sufficient for assembly.
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© 1994 Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt
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Lemas, M.V. et al. (1994). Structural Requirements for the Interaction between the Na+/K+-ATPase α- and β-Subunits. In: Bamberg, E., Schoner, W. (eds) The Sodium Pump. Steinkopff. https://doi.org/10.1007/978-3-642-72511-1_46
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DOI: https://doi.org/10.1007/978-3-642-72511-1_46
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