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Differential aldosterone effects on two epithelial cell pools of [3H]ouabain binding sites

  • Jesper M. Nielsen
  • Peter L. Jørgensen

Abstract

The A6 cell line, derived from the kidney of Xenopus laevis has earlier been shown to respond to aldosterone with an increase in the rate of Na+ transport across the epithelium (8). The rate-limiting steps for transcellular Na+ transport are the passive flux of Na+ into the cytoplasm through amiloride-sensitive Na+-channels in the apical membrane, and the active extrusion of Na+ by Na+/K+-pumps in the basolateral membrane (4). Aldosterone is believed to augment the transcellular Na+ transport by a two-step process. In the early or acute aldosterone-induced response, the molecular activity of existing Na+/K+-pumps in the basolateral membrane is increased in response to an upregulation of the apical membrane Na+ conductance (1), whereas the late or chronic phase of aldosterone action involves upregulation of the amount of Na+/K+-pump sites in the basolateral membrane (3,4).

Keywords

Apical Membrane Basolateral Membrane Ouabain Binding Active Extrusion Passive Flux 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1994

Authors and Affiliations

  • Jesper M. Nielsen
    • 1
  • Peter L. Jørgensen
    • 1
  1. 1.Biomembrane Research Centre, August Krogh InstituteCopenhagen UniversityCopenhagen OEDenmark

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