Differential aldosterone effects on two epithelial cell pools of [3H]ouabain binding sites

  • Jesper M. Nielsen
  • Peter L. Jørgensen


The A6 cell line, derived from the kidney of Xenopus laevis has earlier been shown to respond to aldosterone with an increase in the rate of Na+ transport across the epithelium (8). The rate-limiting steps for transcellular Na+ transport are the passive flux of Na+ into the cytoplasm through amiloride-sensitive Na+-channels in the apical membrane, and the active extrusion of Na+ by Na+/K+-pumps in the basolateral membrane (4). Aldosterone is believed to augment the transcellular Na+ transport by a two-step process. In the early or acute aldosterone-induced response, the molecular activity of existing Na+/K+-pumps in the basolateral membrane is increased in response to an upregulation of the apical membrane Na+ conductance (1), whereas the late or chronic phase of aldosterone action involves upregulation of the amount of Na+/K+-pump sites in the basolateral membrane (3,4).


Apical Membrane Basolateral Membrane Ouabain Binding Active Extrusion Passive Flux 
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Copyright information

© Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1994

Authors and Affiliations

  • Jesper M. Nielsen
    • 1
  • Peter L. Jørgensen
    • 1
  1. 1.Biomembrane Research Centre, August Krogh InstituteCopenhagen UniversityCopenhagen OEDenmark

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