Changes of molecular structure and interaction in the catalytic cycle of sarcoplasmic reticulum Ca2+- ATPase
Infrared difference spectroscopy was used to get more insight into the transport mechanism of sarcoplasmic reticulum Ca2+-ATPase. Several partial reactions were induced directly in the infrared cuvette via photolytic release of ligands or substrates from photolabile precursors (1,2). From the spectra before and after the release a difference spectrum can be calculated that reflects only those changes of infrared absorption which are associated with the catalytic reactions and the triggering photolysis reaction. The accuracy of this method is extremely high, since it avoids possible inaccuracies when spectra of two samples prepared in different enzyme states are compared.
KeywordsSarcoplasmic Reticulum Reaction Step Difference Spectrum Catalytic Cycle Nucleotide Binding
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