Advertisement

Changes of molecular structure and interaction in the catalytic cycle of sarcoplasmic reticulum Ca2+- ATPase

Infrared difference spectra of Ca2+ binding, nucleotide binding, phosphorylation and phosphoenzyme conversion
  • Andreas Barth
  • Holger Georg
  • Werner Kreutz
  • Werner Mäntele

Abstract

Infrared difference spectroscopy was used to get more insight into the transport mechanism of sarcoplasmic reticulum Ca2+-ATPase. Several partial reactions were induced directly in the infrared cuvette via photolytic release of ligands or substrates from photolabile precursors (1,2). From the spectra before and after the release a difference spectrum can be calculated that reflects only those changes of infrared absorption which are associated with the catalytic reactions and the triggering photolysis reaction. The accuracy of this method is extremely high, since it avoids possible inaccuracies when spectra of two samples prepared in different enzyme states are compared.

Keywords

Sarcoplasmic Reticulum Reaction Step Difference Spectrum Catalytic Cycle Nucleotide Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. (1).
    Barth A, Kreutz W and Mäntele M (1990) Molecular changes in the sarcoplasmic reticulum calcium ATPase during catalytic activity. FEBS Lett 277: 147–150.PubMedCrossRefGoogle Scholar
  2. (2).
    Barth A, Mäntele W and Kreutz W (1991) Infrared spectroscopic signals arising from ligand binding and conformational changes in the catalytic cycle of sarcoplasmic reticulum calcium ATPase. Biochim Biophys acta 1057: 115–123.PubMedCrossRefGoogle Scholar

Copyright information

© Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1994

Authors and Affiliations

  • Andreas Barth
    • 1
  • Holger Georg
    • 1
  • Werner Kreutz
    • 1
  • Werner Mäntele
    • 1
  1. 1.Institut für Biophysik und StrahlenbiologieUniversität FreiburgFreiburgGermany

Personalised recommendations