Abstract
In heart and skeletal muscle the sodium pump is crucial for maintenance of membrane potential and cellular integrity. Of the three α-subunit isoforms, α2 and α3 mRNA’s are concentrated in cardiac conduction system (CCS) of normal adult rat (6). We thus studied expression of pump isoform proteins in normal rat heart and skeletal muscle, and in vascular endothelial tissue, using immunofluorescence, immuno-EM, and Western blotting with isoform-specific antibodies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Brines M.L. and R.J. Robbins. Cell-type specific expression of Na,K-ATPase catalytic subunits in cultured neurons and glia: evidence of polarized distribution in neurons. Brain Research (in press).
Kashgarian, M., Biemesderfer, D., Forbush, B., and Caplan, M. (1985) Monoclonal antibody to Na,K-ATPase: immunocytochemical localization along nephron segments. Kidney Int. 28, 899–913
McGrail, K.M., J.M. Phillips, and K.J. Sweadner. Immunofluorescent localization of three Na,K-ATPase isozymes in the rat central nervous system. J. Neuroscience 11: 381–391, 1991.
Pietrini, G., M. Matteoli, G. Banker, and M.J. Caplan. Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture. Proc. Natl. Acad. Sci. 89: 8414–8418, 1992.
Sweadner, K.J. Isozymes of the Na,K-ATPase. Biochim. Biophys. Acta 988: 185–220, 1989.
Zahler R, Brines M, Kashgarian M, Benz, E.J. Jr., Gilmore-Hebert M.: The cardiac conduction system in the rat expresses the α2 and α3 isoforms of the Na,K-ATPase; Proc. Natl. Acad. Sci. 89 (1992) 99–103.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt
About this chapter
Cite this chapter
Zahler, R., Sun, W., Ardito, T., Brines, M., Kashgarian, M. (1994). The α3 isoform protein of the Na+/K+-ATPase is associated with the sites of neuromuscular and cardiac impulse transmission. In: Bamberg, E., Schoner, W. (eds) The Sodium Pump. Steinkopff. https://doi.org/10.1007/978-3-642-72511-1_130
Download citation
DOI: https://doi.org/10.1007/978-3-642-72511-1_130
Publisher Name: Steinkopff
Print ISBN: 978-3-642-72513-5
Online ISBN: 978-3-642-72511-1
eBook Packages: Springer Book Archive