Stimulation by Phorbol 12-Myristate 13-Acetate (PMA) of Na,K-Pump Mediated 86Rb+-Influx Into Human Lymphocytes
The Na,K-pump in cells is a target for both long-term (latency of 1–24 hours) and acute (seconds-minutes) regulation (5,6). In both cases stimulation or decrease in pumping activity is observed. The subject of this paper is the acute up-regulation of the pump, for which a molecular explanation is lacking. We report an acute, stimulating effect of the protein kinase C activator PMA on the pump mediated K+-influx into lymphocytes. Ionomycin has a similar effect, and amiloride does not abolish the stimulation.
KeywordsPreincubation Period Involve Protein Kinase Ionophore Ionomycin Flux Medium Pump Flux
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