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Na,K-ATPase expression in normal and failing human left ventricle

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Cellular and Molecular Alterations in the Failing Human Heart

Summary

The expression of the Na,K-ATPase was studied in both normal and failing human myocardium which was collected within 5 min of cardiac explanation in preparation for orthotopic transplantation or at the time of organ harvest. Abundance of mRNA for all three catalytic α subunits of the Na,K-ATPase was analyzed in samples from patients with end-stage heart failure due to either ischemic or dilated cardiomyopathy, as well as from normal controls. Vanadate facilitated 3H-oubain binding before and after a Digibind wash was analyzed on tissue from a subset of these patients. mRNA analysis demonstrated that all three catalytic Na,K-ATPase α subunits were expressed in human heart and that there was no evidence for change in relative expression or abundance induced by disease. The specific digitalis receptor concentration was 760 ± 58 and 614 ± 47 pmol/g wet weight in the samples from normal and failing hearts, respectively (p = NS). From these studies it can be concluded that, whereas there is a tendency for a decrease in the number of oubain receptors in heart failure, there is no significant alteration in the expression of Na,K-ATPase message or protein caused by chronic heart failure.

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Author information

Authors and Affiliations

  1. Department of Anesthesia, Brigham and Women’s Hospital, Boston, Massachusetts, USA

    Paul D. Allen M.D., Ph.D.

  2. Department of Medicine B, Division of Cardiology, Rigshospitalet, Copenhagen University School of Medicine, Denmark

    T. A. Schmidt & K. Kjeldsen

  3. Department of Medicine, Cardiovascular Division, Brigham and Women’s Hospital, USA

    J. D. Marsh

  4. Department of Anesthesia, Brigham and Women’s Hospital, 75 Francis Street, MA, 02115, Boston, USA

    Paul D. Allen M.D., Ph.D.

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  1. Paul D. Allen M.D., Ph.D.
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  2. T. A. Schmidt
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  3. J. D. Marsh
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  4. K. Kjeldsen
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Editor information

Editors and Affiliations

  1. Innere Medizin III, Kardiologie, Albert-Ludwigs-Universität Medizinische Universitätsklinik, Hugstetter Straβe 55, 7800, Freiburg, Germany

    G. Hasenfuss , Ch. Holubarsch  & H. Just ,  & 

  2. Department of Physiology and Biophysics, University of Vermont, Burlington, VT, 05405, USA

    N. R. Alpert Ph. D.

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© 1992 Dr. Dietrich Steinkopff Verlag GmbH & Co.KG, Darmstadt

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Allen, P.D., Schmidt, T.A., Marsh, J.D., Kjeldsen, K. (1992). Na,K-ATPase expression in normal and failing human left ventricle. In: Hasenfuss, G., Holubarsch, C., Just, H., Alpert, N.R. (eds) Cellular and Molecular Alterations in the Failing Human Heart. Steinkopff. https://doi.org/10.1007/978-3-642-72474-9_7

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  • DOI: https://doi.org/10.1007/978-3-642-72474-9_7

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-642-72476-3

  • Online ISBN: 978-3-642-72474-9

  • eBook Packages: Springer Book Archive

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