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Regulation and possible functional implications of G-protein mRNA expression in nonfailing and failing ventricular myocardium

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Cellular and Molecular Alterations in the Failing Human Heart

Summary

In human end-stage heart failure an increased amount of inhibitory G-protein α-subunits (Giα) is assumed to play a role in desensitization of the adenylyl cyclase signaling pathway. In the present study, northern blot experiments with 32P-labeled cDNA probes in ventricular tissue samples from explanted human hearts revealed that Giα–2- and Giα–3- mRNA are the predominant Giα-mRNA subtypes in human ventricles, whereas Giα–1-mRNA was not detectable. The mRNA for the stimulatory G-protein α-subunit (GSα) consisted of two mRNA sizes. Quantification of mRNA levels revealed a 103 ± 38% increase in Giα–2-mRNA levels in hearts with idiopathic dilative cardiomyopathy (IDC; n = 8), and a 77 ± 25% increase in hearts with ischemic cardiomyopathy (ICM; n = 6) as compared to nonfailing controls (NF, n = 8). In contrast, Giα–3- and GSα-mRNA levels were similar in failing and nonfailing hearts. To investigate whether or not the increased expression of Giα–2-mRNA might be due to chronically elevated catecholamine levels, we determined the influence of a 4-day infusion of isoprenaline (Iso; 2.4 mg/kg d), propranolol (Prop; 9.9 mg/kg d), Iso + Prop or 0.9% NaCl as control (Ctr) on myocardial Giα-mRNA and Giα-protein levels in rats. In Iso-treated rats, hybridization experiments revealed a 49 ± 18% (n = 7) and 27 ± 7% (n = 8) increase in Giα–2 and Giα–3-mRNA, respectively. Pertussis toxin-catalyzed ADP-ribosylation revealed a 22 ± 7% (n = 8) increase in Giprotein as compared to Ctr (n = 8). These alterations were accompanied by an increased potency for the negative inotropic effect (NIE) of carbachol (mean EC50: 0.04 µM vs. 0.28 µM) in the presence of Iso in isolated electrically driven (1 Hz) papillary muscles. Prop itself had no effect, but it antagonized all Iso-induced effects. We conclude that, in human heart failure due to IDC or ICM, increased Giα–2-, but not Giα–3-mRNA levels accompany the increased amount of Giα-protein, suggesting that this increase is at least in part due to increased de novo synthesis. The experiments in rats demonstrated that chronic β-adrenergic stimulation leads to an increased expression of Giα-mRNA and -protein, and to an enhanced potency of the negative inotropic effect of muscarinic agonists. These results support the hypothesis that increased expression of Giα might play a pathophysiological role in human end-stage heart failure as a process of adaptation to an increased adrenergic drive.

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Authors and Affiliations

  1. Abteilung Allgemeine Pharmakologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany

    Dr. med. T. Eschenhagen, U. Mende, M. Nose, W. Schmitz, H. Scholz, J. Schulte am Esch, R. Sempell, A. Warnholtz & J.-M. Wüstel

  2. Abteilung Allgemeine Pharmakologie, Universitäts-Krankenhaus Eppendorf, Martinistr, 52, 2000, Hamburg 20, Germany

    Dr. med. T. Eschenhagen

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  1. Dr. med. T. Eschenhagen
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  2. U. Mende
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  3. M. Nose
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  4. W. Schmitz
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  5. H. Scholz
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  6. J. Schulte am Esch
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  7. R. Sempell
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  8. A. Warnholtz
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  9. J.-M. Wüstel
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Editors and Affiliations

  1. Innere Medizin III, Kardiologie, Albert-Ludwigs-Universität Medizinische Universitätsklinik, Hugstetter Straβe 55, 7800, Freiburg, Germany

    G. Hasenfuss , Ch. Holubarsch  & H. Just ,  & 

  2. Department of Physiology and Biophysics, University of Vermont, Burlington, VT, 05405, USA

    N. R. Alpert Ph. D.

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© 1992 Dr. Dietrich Steinkopff Verlag GmbH & Co.KG, Darmstadt

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Eschenhagen, T. et al. (1992). Regulation and possible functional implications of G-protein mRNA expression in nonfailing and failing ventricular myocardium. In: Hasenfuss, G., Holubarsch, C., Just, H., Alpert, N.R. (eds) Cellular and Molecular Alterations in the Failing Human Heart. Steinkopff. https://doi.org/10.1007/978-3-642-72474-9_4

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  • DOI: https://doi.org/10.1007/978-3-642-72474-9_4

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-642-72476-3

  • Online ISBN: 978-3-642-72474-9

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