Advertisement

Storage and secretion of atrial natriuretic peptide (ANP) by cultured atrial and ventricular cells from the neonatal rat

  • T. Brand
  • H. Jockusch
  • G. Rippegather
  • W. G. Forssmann
Conference paper

Abstract

In the heart of the neonatal rat, myoendocrine ANP producing cells are located in the right and left atria and in the conduction tissue mainly of the left ventricle (2,10,11). When minced atrial and ventricular tissues from neonatal rats were transplanted to ectopic sites and analyzed after several weeks, strong ANP immunoreactivity was found only in atrial grafts (6). In cell culture atrial cells were ANP-immunoreactive but ventricular cells were not (3).

Keywords

Atrial Natriuretic Peptide Atrial Natriuretic Factor Atrial Cell Ventricular Cell Immunochemical Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Arjamaa, O., Vuolteenaho, O. (1985) Sodium ion stimulates the release of atrial natriuretic polypeptides (ANP) from rat atria. Biochem Biophys Res Commun 132: 375–381PubMedCrossRefGoogle Scholar
  2. 2.
    Back, H., Stumpf, W.E., Ando, E., Nokihara, K., Forssmann, W.G. (1986) Immunocytochemical evidence for CDD/ANP-like peptides in strands of myoendocrine cells associated with the ventricular conduction system of the rat heart. Anat Embryol 175: 223–226PubMedCrossRefGoogle Scholar
  3. 3.
    Bloch, K.D., Seidmann, J.G., Naftilan, J.D., Fallon, J.T., Seidman, C.E. (1986) Neonatal atria and ventricular secrete atrial natriuretic factor via tissue-specific secretory pathways. Cell 47: 695–702PubMedCrossRefGoogle Scholar
  4. 4.
    Brand, Th., Jockusch, H. (1987) Differential properties of rat atrial and ventricular muscle cells in culture. Eur J Cell Biol 43: 8Google Scholar
  5. 5.
    Forssmann, W.G., Pickel, V., Reinecke, M., Hock, D., Metz, J. (1981) Immunohistochemistry and immunocytochemistry of nervous tissue. In: Heym, Ch., Forssmann, W.G. (eds) Techniques in neuro anatomical research. Springer, Berlin Heidelberg New York, pp 171–205Google Scholar
  6. 6.
    Jockusch, H., Füchtbauer, E.M., Füchtbauer, A., Léger, J.J., Léger, J., Maldonado, C.A., Forssmann, W.G. (1986) Long term expression of isomyosins and myoendocrine functions in ectopic grafts of atrial tissue. Proc Nat Acad Sci USA 83: 6697–6701CrossRefGoogle Scholar
  7. 7.
    Jourdon, P. (1980) Ultrastructure and electrically activity of newborn rat heart reaggregates. Biol Cell 37: 149–154Google Scholar
  8. 8.
    Lang, R.E., Thölken, H., Ganten, D., Luft, F.C., Ruoskaoh, H., Unger, Th. (1985) Atrial natriuretic factor: a circulating hormone stimulated by volume loading. Nature 314: 264–266PubMedCrossRefGoogle Scholar
  9. 9.
    Lompré, A.M., Poggioli, J., Vassort, G. (1979) Maintenance of fast Na-channels during primary culture of chick heart cells. J Mol Cell Cardiol 11: 813–825PubMedCrossRefGoogle Scholar
  10. 10.
    Thompson, R.P., Simpson, J.A.V., Currie, M.G. (1986) Atriopeptin distribution in the developing rat heart. Anat Embryol 175: 227–233PubMedCrossRefGoogle Scholar
  11. 11.
    Toshimori, H., Toshimori, K., Oura, C., Matsuo, H. (1987) Immunohistochemical study of atrial natriuretic polypeptides in the embryonic, foetal and neonatal rat heart. Cell Tissue Res 248–633Google Scholar

Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1989

Authors and Affiliations

  • T. Brand
    • 1
  • H. Jockusch
    • 1
  • G. Rippegather
    • 2
  • W. G. Forssmann
    • 2
  1. 1.Developmental Biology UnitUniversity of BielefeldBielefeldGermany
  2. 2.Department of Anatomy and Cell BiologyUniversity of HeidelbergHeidelbergGermany

Personalised recommendations