Factors affecting the viability of cryopreserved allograft heart valves
Allograft heart valves have been used for more than 25 years as replacements for diseased aortic valves and repair of congenital abnormalities. In many instances, particularly paediatric surgery, it is the valve of choice due to its non-obstructive flow, relative freedom from calcification and thromboembolism without anticoagulation therapy. In order to permit valve size matching for recipients, it is necessary to find methods of storage which will maintain cellular viability. The best method which allows infinite, convenient storage is cryopreservation.
KeywordsGlycerol DMSO Ethylene Glycol Adenosine Proline
Unable to display preview. Download preview PDF.
- 3.Bank HL, Schmehl MK, Brockbank KGM (1988) Endothelial and fibroblast viability assays for tissue allografts. In: Yankah C A et al. (eds) Current concepts on the use of aortic and pulmonary allografts for heart valve substitutes, Berlin 7–9 September 1987. Steinkopff, DarmstadtGoogle Scholar
- 11.LoGrippo GA, Overhulse PR, Szilagyi DC, Hartman FW (1955) Procedure for the sterilization of arterial homografts with beta-propiolactone. Lab Invest 4: 217Google Scholar
- 12.Mazur P (1965) Causes of injury in frozen and thawed cells. Fed Proc 24 (Suppl 15): 175Google Scholar
- 15.Meeker IA Jr, Gross RE (1951) Sterilization of frozen arterial grafts by high voltage cathode-ray irradiation. Surgery 63: 45Google Scholar
- 17.O’Brien MF, Stafford G, Gardner M, Pohlner P, McGiffin D, Johnston N, Brosnan A, Duffy P (1987) The viable cryopreserved allograft aortic valve. J Cardiac Surg 2 (Suppl): 153–167Google Scholar
- 24.Bank HL, Brockbank KGM (1987) Basic principles of cryobiology. J Cardiac Surg 2 (Suppl): 137Google Scholar