Modular Fusion Proteins for Receptor-mediated Gene Delivery
The development of vectors for target-cell specific gene delivery is a major goal of gene therapeutic strategies. Thereby non-viral gene delivery vectors are gaining increasing interest . Progress has been made in the understanding how individual activities of viruses can be mimicked and methodologies have been developed which allow to combine different functions required for gene transfer into an artificial complex. An attractive approach for the design of such modular self-assembling systems for gene delivery is based on fusion proteins engineered to incorporate in a single polypeptide chain several cooperating functions [2–4]. Each of these domains can account for a distinct activity required for DNA-binding, cell recognition and intracellular delivery. Such fusion proteins in contrast to similar chemical conjugates can be produced in suitable expression systems in their final form and the resulting products are generally homogeneous in their composition.
KeywordNon-viral gene delivery fusion protein Pseudomonasexotoxin A diphtheria toxin GAL4 ErbB2
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