Surgical Technique for Skin Transplantation in Mice
Much of the knowledge about the immunological and pathological mechanisms of graft rejection has been gained by experimental studies in allogeneic skin transplantation in mice. Since its detailed description by Billingham and Meda-war in 1951, it has been widely used to study the T cell mediated immune response of allograft rejection [1, 2]. The surgical procedure is easy to learn, does not need microsurgical equipment, and can be performed numerous times in a short period. Acute skin graft rejection is a striking and reproducible immunological reaction, and with some experience the time point of rejection can be determined accurately. This chapter describes the techniques of skin transplantation in mice currently used in our laboratory and discusses its applications.
KeywordsAllograft Rejection Graft Rejection Minor Histocompatibility Antigen Native Skin Tail Skin
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