Skip to main content
SpringerLink
Log in

UV and blue light signal transduction in the regulation of flavonoid biosynthesis gene expression in Arabidopsis

  • Conference paper
Cellular Integration of Signalling Pathways in Plant Development

Part of the book series: NATO ASI Series ((ASIH,volume 104))

Abstract

Biochemical and genetic approaches in Arabidopsis have produced new insights into the photoreceptors and signal transduction processes involved in the regulation of gene expression by UV and blue light. Several distinct photoreceptors mediate the effects of UV-B, UV-A and blue light on the expression of the gene encoding the flavonoid biosynthesis enzyme chalcone synthase (CHS). Information on the signal transduction components involved in the induction of CHS gene expression has been obtained in experiments with an Arabidopsis cell culture. Experiments with intact plants indicate that interactions between phototransduction pathways maximise CHS expression. Mutants altered in negative regulators of CHS transcription in response to UV and blue light have been isolated.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ahmad M. and Cashmore A.R. (1993) HY4 gene of A. thaliana encodes a protein with the characteristics of a blue-light photoreceptor. Nature 366, 162–166.

    Article  CAS  PubMed  Google Scholar 

  • Ahmad M. and Cashmore A.R. (1996) Seeing blue: the discovery of cryptochrome. Plant Mol Biol. 30, 851–861.

    Article  CAS  PubMed  Google Scholar 

  • Ahmad M. and Cashmore A.R. (1997) The blue-light receptor cryptochrome 1 shows functional dependence on phytochrome A or phytochrome B in Arabidopsis thaliana. Plant J. 11, 421–427.

    Article  CAS  PubMed  Google Scholar 

  • Ahmad M., Lin C. and Cashmore A.R. (1995) Mutations throughout an Arabidopsis blue-light photoreceptor impair blue-light-responsive anthocyanin accumulation and inhibition of hypocotyl extension. Plant J. 8, 653–658.

    Article  CAS  PubMed  Google Scholar 

  • Allen G.J., Muir S.R. and Sanders D. (1995) Release of Ca2+ from individual plant vacuoles by both InsP3 and cyclic ADP-ribose. Science 268, 735–737.

    Article  CAS  PubMed  Google Scholar 

  • Assmann S.M., Simoncini L. and Schroeder J.I. (1985) Blue light activates electrogenic ion pumping in guard cell protoplasts of Vicia faba. Nature 318, 285–287.

    Article  CAS  Google Scholar 

  • Batschauer A., Ehmann B. and Schäfer E. (1991) Cloning and characterisation of a chalcone synthase gene from mustard and its light-dependent expression. Plant Mol. Biol. 16, 175–185.

    Article  CAS  PubMed  Google Scholar 

  • Batschauer A., Rocholl M., Kaiser T., Nagatani A., Furuya M. and Schäfer E. (1996) Blue and UV-A light-regulated CHS expression in Arabidopsis independent of phytochrome A and phytochrome B. Plant J. 9, 63–69.

    Article  CAS  Google Scholar 

  • Bowler C., Neuhaus G., Yamagata H. and Chua N-H. (1994a) Cyclic GMP and calcium mediate phytochrome phototransduction. Cell 77, 73–81.

    Article  CAS  PubMed  Google Scholar 

  • Bowler C., Yamagata H., Neuhaus G. and Chua N-H. (1994b) Phytochrome signal transduction pathways are regulated by reciprocal control mechanisms. Genes & Development 8, 2188–2202.

    Article  CAS  Google Scholar 

  • Cho M.H. and Spalding E.P. (1996) An anion channel in Arabidopsis hypocotyls activated by blue light. Proc. Natl Acad. Sci. USA 93, 8134–8138.

    Article  CAS  PubMed  Google Scholar 

  • Christie J.M. and Jenkins G.I. (1996) Distinct UV-B and UV-A/blue light signal transduction pathways induce chalcone synthase gene expression in Arabidopsis cells. Plant Cell 8, 1555–1567.

    Article  CAS  PubMed  Google Scholar 

  • Dixon R.A. and Paiva N.L. (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7, 1085–1097.

    Article  CAS  PubMed  Google Scholar 

  • Feinbaum R.L. and Ausubel F.M. (1988) Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol. Cell Biol 8, 1985–1992.

    CAS  PubMed  Google Scholar 

  • Feinbaum R.L., Storz G. and Ausubel F.M. (1991) High intensity and blue light regulated expression of chimeric chalcone synthase genes in transgenic Arabidopsis thaliana plants. Mol. Gen. Genet. 226, 449–456.

    Article  CAS  PubMed  Google Scholar 

  • Feldbrugge M., Hahlbrock K. and Weisshaar B. (1996) The transcriptional regulator CPRF1: expression analysis and gene structure. Mol Gen. Genet. 251, 619–627.

    CAS  PubMed  Google Scholar 

  • Feldbrugge M., Sprenger M., Hahlbrock K. and Weisshaar B. (1997) PcMYBl, a novel plant protein containing a DNA-binding domain with one MYB repeat, interacts in vivo with a light-regulatory promoter unit. Plant J. 11, in press.

    Google Scholar 

  • Frohnmeyer H., Ehmann B., Kretsch T., Rocholl M., Harter. K., Nagatani A., Furuya M., Batschauer A., Hahlbrock K. and Schäfer E. (1992) Differential usage of photoreceptors for chalcone synthase gene expression during plant development. Plant J. 2, 899–906.

    Article  CAS  Google Scholar 

  • Fuglevand G., Jackson J.A. and Jenkins G.I. (1996) UV-B, UV-A and blue light signal transduction pathways interact synergistically to regulate chalcone synthase gene expression in Arabidopsis. Plant Cell 8, 2347–2357.

    Article  CAS  PubMed  Google Scholar 

  • Harter K., Kircher S., Frohnmeyer H., Krenz M., Nagy F. and Schäfer, E. (1994) Light-regulated modification and nuclear translocation of cytosolic G-box binding factors in parsley. Plant Cell 6, 545–559.

    Article  CAS  PubMed  Google Scholar 

  • Jackson J.A., Fuglevand G., Brown B.A., Shaw M.J. and Jenkins G.I. (1995) Isolation of Arabidopsis mutants altered in the light-regulation of chalcone synthase gene expression using a transgenic screening approach. Plant J. 8, 369–380.

    Article  CAS  PubMed  Google Scholar 

  • Jackson J.A. and Jenkins G.I. (1995) Extension growth responses and flavonoid biosynthesis gene expression in the Arabidopsis hy4 mutant. Planta 197, 233–239.

    Article  CAS  PubMed  Google Scholar 

  • Jenkins G.I., Christie J.M., Fuglevand G., Long J.C. and Jackson J.A. (1995) Plant responses to UV and blue light: biochemical and genetic approaches. Plant Sci. 112, 117–138.

    Article  CAS  Google Scholar 

  • Kaiser T., Emmler K., Kretsch T., Weisshaar B., Schäfer E. and Batschauer, A. (1995) Promoter elements of the mustard CHS1 gene are sufficient for light-regulation in transgenic plants. Plant Mol Biol. 28, 219–229.

    Article  CAS  PubMed  Google Scholar 

  • Kaufman L.S. (1993) Transduction of blue light signals. Plant Physiol 102, 333–337.

    CAS  PubMed  Google Scholar 

  • Koornneef M., Rolff E. and Spruit C.J.P. (1980) Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana. Z. Pflanzenphysiol 100, 147–160.

    Google Scholar 

  • Kubasek W.L., Shirley B.W., McKillop A., Goodman H.M., Briggs W.R. and Ausubel F.M. (1992) Regulation of flavonoid biosynthetic genes in germinating Arabidopsis seedlings. Plant Cell 4, 1229–1236.

    Article  CAS  PubMed  Google Scholar 

  • Lin C., Ahmad M. and Cashmore A.R. (1996) Arabidopsis cryptochrome 1 is a soluble protein mediating blue-light-dependent regulation of plant growth and development. Plant J. 10, 893–902.

    Article  CAS  PubMed  Google Scholar 

  • Lin C., Robertson D.E., Ahmad M., Raibekas R.A., Jörns S., Dutton L. and Cashmore A.R. (1995) Association of flavin adenine dinucleotide with the Arabidopsis blue light receptor CRY1. Science 269, 968–970.

    Article  CAS  PubMed  Google Scholar 

  • Liscum E. and Briggs W.R. (1995) Mutations in the NPH1 locus of Arabidopsis disrupt the perception of phototropic stimuli. Plant Cell 7, 473–485.

    Article  CAS  PubMed  Google Scholar 

  • Liscum E. and Hangarter R.P. (1994) Mutational analysis of blue-light sensing in Arabidopsis. Plant, Cell & Env. 17, 639–648.

    Article  CAS  Google Scholar 

  • Malhotra K., Kim S-T., Batschauer A., Dawut L. and Sancar A. (1995) Putative blue-light photoreceptors from Arabidopsis thaliana and Sinapis alba with a high degree of sequence homology to DNA photolyase contain the two photolyase cofactors but lack DNA repair activity. Biochemistry 34, 6892–6899.

    Article  CAS  PubMed  Google Scholar 

  • Mol J., Jenkins G.I., Schäfer E. and Weiss D. (1996) Signal perception, transduction, and gene expression involved in anthocyanin biosynthesis. Crit. Rev. Plant Sci. 15, 525–557.

    CAS  Google Scholar 

  • Neuhaus G, Bowler C., Kern R. and Chua N-H. (1993) Calcium/calmodulin-dependent and -independent phytochrome signal transduction pathways. Cell 73, 937–952.

    Article  CAS  PubMed  Google Scholar 

  • Nishizaki Y. (1994) Vanadate and dicyclohexylcarbodiimide inhibit the blue light- induced depolarisation of the membrane in pulvinar motor cells of Phaseolus. Plant & Cell Physiol 35, 841–844.

    CAS  Google Scholar 

  • Ohl S., Hahlbrock K. and Schäfer E. (1989) A stable blue-light-derived signal modulates ultraviolet-light-induced activation of the chalcone synthase gene in cultured parsley cells. Planta 177, 228–236.

    Article  CAS  Google Scholar 

  • Peters J.L., Schreuder M.E.L., Verduin S.J.W. and Kendrick R.E. (1992) Physiological characterization of a high-pigment mutant of tomato. Photochem. Photobiol. 56, 75–82.

    Article  CAS  Google Scholar 

  • Schulze-Lefert P., Becker-Andre M., Schulz W., Hahlbrock K. and Dangl J.L. (1989) Functional architecture of the light-responsive chalcone synthase promoter from parsley. Plant Cell 1, 707–714.

    Article  CAS  PubMed  Google Scholar 

  • Shimazaki K., Ino M. and Zeiger E. (1986) Blue light-dependent proton extrusion by guard-cell protoplasts of Vicia faba. Nature 319, 324–326.

    Article  CAS  Google Scholar 

  • Short T.W. and Briggs W.R. (1994) The transduction of blue light signals in higher plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 45, 143–171.

    Article  CAS  Google Scholar 

  • Spalding E.P. and Cosgrove D.J. (1989) Large plama-membrane depolarization precedes rapid blue-light-induced growth inhibition in cucumber. Planta 178, 407–410.

    Article  CAS  PubMed  Google Scholar 

  • Spalding E.P. and Cosgrove D.J. (1992) Mechanism of blue-light-induced plasma- membrane depolarisation in etiolated cucumber hypocotyls. Planta 188, 199–205.

    Article  CAS  PubMed  Google Scholar 

  • Stapleton A.E. (1992) Ultraviolet radiation and plants: burning questions. Plant Cell 4, 1353–1358.

    Article  PubMed  Google Scholar 

  • Tevini M. and Teramura A.H. (1989) UV-B effects on terrestrial plants. Photochem. Photobiol. 50, 479–487.

    Article  CAS  Google Scholar 

  • Weisshaar B., Armstrong G.A., Block A., da Costa e Silva O. and Hahlbrock K. (1991) Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light- responsiveness. Embo J. 10, 1777–1786.

    CAS  Google Scholar 

  • Whitelam G.C. and Harberd, N.P. (1994) Action and function of phytochrome family members revealed through the study of mutant and transgenic plants. Plant, Cell & Env. 17, 615–625.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Bower Building, Glasgow, G12 8QQ, UK

    Gareth I. Jenkins

Authors
  1. Gareth I. Jenkins
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Biology, University of Padua, Via Colombo 3, I-35121, Padua, Italy

    Fiorella Lo Schiavo

  2. Boyce Thompson Institute for Plant Research and Section of Genetics and Development, Cornell University, Tower Road, 14853-1801, Ithaca, NY, USA

    Robert L. Last

  3. Unità di Nutrizione Sperimentale, Istituto Nazionale della Nutrizione, Via Ardeatina 546, I-00178, Rome, Italy

    Giorgio Morelli

  4. Department of Energy and Plant Research Laboratory, Michigan State University, 48824-1312, East Lansing, MI, USA

    Natasha V. Raikhel

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Jenkins, G.I. (1998). UV and blue light signal transduction in the regulation of flavonoid biosynthesis gene expression in Arabidopsis . In: Lo Schiavo, F., Last, R.L., Morelli, G., Raikhel, N.V. (eds) Cellular Integration of Signalling Pathways in Plant Development. NATO ASI Series, vol 104. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72117-5_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-72117-5_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72119-9

  • Online ISBN: 978-3-642-72117-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation