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Use of transient expression in plants for the study of the “gene-for-gene” interaction

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Cellular Integration of Signalling Pathways in Plant Development

Part of the book series: NATO ASI Series ((ASIH,volume 104))

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Abstract

One of the common responses in disease resistance defined by the “gene-for-gene” interaction is the hypersensitive response (HR), which is a rapid and localized cell death of plants at the site of infection. This specific cell death was used to develop a biolistic transient expression assay for the resistance response. If the transiently expressed gene causes the HR, the expression level of a cointroduced reporter gene decreases due to the rapid death of the cell. Using this assay, it was shown that expression of the Pseudomonas syringae avirulence genes, avrRpt2 or avrB, in Arabidopsis thaliana can elicit the HR when the plants carry the corresponding resistance gene, RPS2 or RPM1, respectively. This indicates that the avirulence genes are the only bacterial factors that are required to elicit the specific resistance response as long as the avirulence gene products are localized properly. This observation and others strongly suggest that the molecular recognition of pathogen attack occurs inside of plant cells for these combinations of avirulence gene and resistance gene. RPS2 and RPM1 are members of NBS-LRR class of resistance genes. NBS-LRR proteins could be cytoplasmic receptors for specific signal molecules from avirulent pathogens. We propose a speculative model to view different classes of resistance gene products from a unified perspective.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD, 21250, USA

    Fumiaki Katagiri & R. Todd Leister

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  1. Fumiaki Katagiri
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  2. R. Todd Leister
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Editors and Affiliations

  1. Department of Biology, University of Padua, Via Colombo 3, I-35121, Padua, Italy

    Fiorella Lo Schiavo

  2. Boyce Thompson Institute for Plant Research and Section of Genetics and Development, Cornell University, Tower Road, 14853-1801, Ithaca, NY, USA

    Robert L. Last

  3. Unità di Nutrizione Sperimentale, Istituto Nazionale della Nutrizione, Via Ardeatina 546, I-00178, Rome, Italy

    Giorgio Morelli

  4. Department of Energy and Plant Research Laboratory, Michigan State University, 48824-1312, East Lansing, MI, USA

    Natasha V. Raikhel

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© 1998 Springer-Verlag Berlin Heidelberg

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Katagiri, F., Leister, R.T. (1998). Use of transient expression in plants for the study of the “gene-for-gene” interaction. In: Lo Schiavo, F., Last, R.L., Morelli, G., Raikhel, N.V. (eds) Cellular Integration of Signalling Pathways in Plant Development. NATO ASI Series, vol 104. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72117-5_27

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  • DOI: https://doi.org/10.1007/978-3-642-72117-5_27

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72119-9

  • Online ISBN: 978-3-642-72117-5

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