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High-Resolution Three-Dimensional Imaging with an X-Ray Microscope

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Abstract

The Göttingen transmission X-ray microscope (TXM) at BESSY has been used for threedimensional imaging. As a first test-object sheaths of the bacteria Leptothrix Ochracea have been visualized. 33 tilted-view images have been taken at tilt angles between 0 and 160 degrees in steps of 5 degrees. Before reconstruction the images were flat-field corrected and aligned. The rotation axis was determined and the images were rotated accordingly. For tomographic reconstruction a fast MART (Multiplicative Algebraic Reconstruction Technique) algorithm was used. In the recon-structed volume structures of sizes well below 100 nm are made visible.

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References

  1. G. Schmahl, D. Rudolph, B. Niemann, P. Guttmann, J. Thieme, G. Schneider, C. David, M. Diehl and T. Wilhein: X-Ray Microscopy Studies. Optik 93, pp. 95–102, (1993).

    Google Scholar 

  2. G. Schmahl, D. Rudolph; B. Niemann, P. Guttmann, J. Thieme and G. Schneider: Rontgenmikroskopie. Naturwissenschaften 83, (1996), pp. 61–70.

    Article  ADS  Google Scholar 

  3. V.V. Aristov and A. I. Erko (Eds.): X-Ray Microscopy IV. Proceedings of the 4-th International Conference, September 20–24, 1993. Chernogolovka, Russia, 1994.

    Google Scholar 

  4. Niemann, G. Schneider, P. Guttmann, D. Rudolph and G. Schmahl: The New Göttingen X-Ray Microscope with Object Holder in Air for Wet Specimens. in: [3] pp. 66–75.

    Google Scholar 

  5. W.S. Haddad, I. McNulty, JE. Trebes, E.H. Anderson, R.A. Levesque, L. Yang. Ultrahigh-Resolution X-ray Tomography. Sciene, vol. 266, 18′Nov. (1994), pp.1213–1215.

    Article  ADS  Google Scholar 

  6. J. Lehr: 3D X-Ray Microsopy: High-Resolution Stereo-Imaging with the Göttingen X-Ray Microscope at BESSY. Zoological Studies 34, Supplement (1995), pp. 137–138.

    Google Scholar 

  7. Rosenfeld and A.C. Kak: Digital Picture Processing. Academic press (1982). second edition, volume 1, pp. 353–430.

    Google Scholar 

  8. Schneider, B. Niemann, P. Guttmann, D. Rudolph and G. Schmahl: Cryo xRay Microscopy. Synchrotron Radiation News, vol. 8, No.3 (1995).

    Google Scholar 

  9. D. Verhoeven: Limited-data-computed tomography algorithms for the physical sciences. Applied Optics, vol. 32, No. 20, 10 July (1993), pp. 3736–3754.

    Article  ADS  Google Scholar 

  10. G Lehr: 3D X-Ray Microscopy: High-Resolution Tomographic Imaging of Mineral Sheaths of Bacteria Leptothrix Ochracea. Optik. (in press)

    Google Scholar 

  11. Thieme, T. Wilhein, P. Guttmann, J. Niemeyer, K.-H. Jacob, S. Dietrich: Direct Visualization ofIron and Manganese Accumulating Microorganisms by X-Ray Microscopy. in: [3], pp.152–156.

    Google Scholar 

  12. T. Wilhein: Gedünnte CCDs: Charakterisierung und Anwendungen im Bereich weicher Röntgenstrahlung. Verlag Shaker, Dissertation Universitat Göttingen, (1994).

    Google Scholar 

  13. R.C. Weast (ed.): CRC Handbook of Chemistry and Physics. CRC Press, Boca Raton, Florida (1988).

    Google Scholar 

  14. G. Schneider: Investigations of Soft X-Radiation Induced Structural Changes in Wet Biological Objects. in: [Aristov V.V, A.L. Erko (eds.), 1994], pp. 181–195.

    Google Scholar 

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© 1998 Springer-Verlag Berlin Heidelberg

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Lehr, J. (1998). High-Resolution Three-Dimensional Imaging with an X-Ray Microscope. In: Thieme, J., Schmahl, G., Rudolph, D., Umbach, E. (eds) X-Ray Microscopy and Spectromicroscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72106-9_8

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  • DOI: https://doi.org/10.1007/978-3-642-72106-9_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72108-3

  • Online ISBN: 978-3-642-72106-9

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