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X-Ray Microscopy and Spectromicroscopy pp 25–34Cite as

Cryo X-Ray Microscopy Experiments with the X-Ray Microscope at BESSY

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Abstract

The transmission X-ray microscope (TXM) at the electron storage ring BESSY was used to image frozen-hydrated objects at cryogenic temperatures and atmospheric pressure. After shock freezing in liquid ethane different initially living biological objects such as cells, chromosomes and algae were investigated at 2.4 nm wavelength in amplitude contrast mode. The high X-ray absorption of cellular structures and the 10-fold lower absorption of frozen water yield natural element contrast. The X-ray images show details inside the biological objects down to 30 nm size, which is about one order of magnitude better than conventional visible light microscopy.

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References

  1. H. Wolter, Ann. Physik, 6.Folge, Bd.10, (1952) 94–114

    Article  MATH  Google Scholar 

  2. G. Schmahl, D. Rudolph, G. Schneider, P. Guttmann, B. Niemann, Optik 97, No.4, (1994) 181–182

    Google Scholar 

  3. G. Schmahl, D. Rudolph, P. Guttmann, G. Schneider, J. Thieme, and B. Niemann, Rev. Sci. Instrum. 66 (2), (February 1995) 1282–1286

    Article  ADS  Google Scholar 

  4. G. Schmahl, D. Rudolph, in: X-Ray Microscopy Instrumentation and Biological Applications eds. P. C. Cheng, G. J. Jan, Springer-Verlag, (1987) 231–238

    Google Scholar 

  5. J. Lehr, Optik 104, No.4, (1997) 166–170

    Google Scholar 

  6. C. Dinges, Dissertation, Technische Hochschule Darmstadt, (1996)

    Google Scholar 

  7. D. Sayre, J. Kirz, R. Feder, D. M. Kim, and E. Spiller, Ultramicroscopy 2, (1997) 337–349

    Article  Google Scholar 

  8. D. Rudolph, G. Schmahl and B. Niemann, in: Modern Microscopies, Techniques and Applications, A. Michette, and P. Duke eds., Plenum Press, (1990) 59–67

    Chapter  Google Scholar 

  9. G. Schneider, Dissertation, University of Göttingen, (1992)

    Google Scholar 

  10. G. Schneider, in X-ray Microscopy IV, V. V. Aristov and A.I. Erko (Eds.), Bogorodskii Pechatnik Publishing Company, Chernogolovka, Moscow Region, (1994) 181–195

    Google Scholar 

  11. L. Reimer: Transmission Electron Microscopy, Springer Series in Optical Sciences Vol.36, (1989) 450–451

    Article  Google Scholar 

  12. G. Schneider and B. Niemann, X-Ray Science, 2, Summer 1994 newsletter, Center for X-ray science, King’s College, London, (1994) 8–9

    Google Scholar 

  13. G. Schneider, B. Niemann, P. Guttmann, D. Rudolph and G. Schmahl, Synchrotron Radiation News, Vol. 8, No.3, (1995) 19–28

    Article  Google Scholar 

  14. G. Schmahl and D. Rudolph, Optik 29, (1969) 577–585

    ADS  Google Scholar 

  15. B. Niemann, D. Rudolph, and G. Schmahl, Optics Communications 12, (1974) 160–163

    Article  ADS  Google Scholar 

  16. T. Wilhein, D. Rothweiler, A. Tusche, W. Meyer-Ilse, in: X-ray Microscopy IV, Aristov, V. V. & Erko, A.I. (Eds.), Bogorodskii Pechatnik Publishing Company, Chernogolovka, Moscow Region, (1994) 470–474

    Google Scholar 

  17. B. Niemann, G. Schneider, P. Guttmann, D. Rudolph and G. Schmahl, in Xray Microscopy IV, V. V. Aristov and A.I. Erko (Eds.), Bogorodskii Pechatnik Publishing Company, Chernogolovka, Moscow Region, (1994) 66–75

    Google Scholar 

  18. P. Echlin: Low-Temperature Microscopy and Analysis, Plenum Press, New York, (1992)

    Book  Google Scholar 

  19. G. Schneider, T. Schliebe, and H. Aschoff, J. Vac. Sci. Technol. B 13(6), (Nov/Dez 1995) 2809–2812

    Article  Google Scholar 

  20. T. Schliebe, G. Schneider and H. Aschoff, Microelectronic Engineering 30, (1996) 523–516

    Article  Google Scholar 

  21. G. Schneider, G. Schmahl, T. Schliebe, M. Peuker, and P. Guttmann, (1996) this conference

    Google Scholar 

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Authors and Affiliations

  1. Forschungseinrichtung Röntgenphysik, Georg-August-Universität Göttingen, Geiststraße 11, D-37073, Göttingen, Germany

    G. Schneider & B. Niemann

Authors
  1. G. Schneider
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  2. B. Niemann
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Editors and Affiliations

  1. Forschungseinrichtung Röntgenphysik, Georg-August-Universität Göttingen, Geiststrasse 11, D-37073, Göttingen, Germany

    Jürgen Thieme , Günter Schmahl  & Dietbert Rudolph ,  & 

  2. Experimentelle Physik II, Universität Würzburg, Am Hubland, D-97074, Würzburg, Germany

    Eberhard Umbach

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© 1998 Springer-Verlag Berlin Heidelberg

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Schneider, G., Niemann, B. (1998). Cryo X-Ray Microscopy Experiments with the X-Ray Microscope at BESSY. In: Thieme, J., Schmahl, G., Rudolph, D., Umbach, E. (eds) X-Ray Microscopy and Spectromicroscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72106-9_3

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  • DOI: https://doi.org/10.1007/978-3-642-72106-9_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72108-3

  • Online ISBN: 978-3-642-72106-9

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