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A Perspective on Biological X-Ray and Electron Microscopy

  • Chris Jacobsen
  • Robin Medenwaldt
  • Shawn Williams

Abstract

We consider image contrast for electron microscopy of thick hydrated biological specimens, allowing for the use of phase contrast and energy filtering. This allows us to gain perspective on the relative roles of electron and soft X-ray microscopes. Radiation dose is found to depend strongly on ice thickness, with electrons offering lower dose if the ice thickness is less than about 500 nm, and x rays offering lower dose for thicker ice layers.

Keywords

Elastic Scattering Inelastic Scattering Electron Energy Loss Spectroscopy Objective Aperture Energy Filter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Chris Jacobsen
    • 1
  • Robin Medenwaldt
    • 2
  • Shawn Williams
    • 3
  1. 1.Department of PhysicsSUNY Stony BrookStony BrookUSA
  2. 2.Institute for Storage Ring FacilitiesUniversity of AarhusAarhus CDenmark
  3. 3.Boyer Center for Molecular MedicineYale UniversityNew HavenUSA

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