Apoptosis and the Cytopathic Effects of Reovirus
Reoviruses are cytolytic viruses which cause the death of infected cells in vitro and in vivo. The relationship between events involved in the production of virus and death of the infected cell is not clearly understood. Studies of virus-induced biochemical and morphological changes in cells, i.e., cytopathic effects, have begun to define both viral and cellular factors involved in virus-induced cell death. The description and definition of apoptosis as a mechanism of cell death distinct from necrosis has provided a framework for elucidating the roles of these viral and cellular factors in the cell death process. The demonstration that reovirus induces apoptosis in vitro and in vivo identifies apoptosis as an important component of reovirus-induced cytopathic effects and tissue injury. In this chapter we review the current data describing reovirus-induced apoptosis and propose that many of the previously described cytopathic effects of reovirus infection represent events which initiate or comprise steps in the apoptotic process.
KeywordsMDCK Cell Cytopathic Effect Viral Growth Reassortant Virus Outer Capsid Protein
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- Boise LH, Gottschalk AR, Quintans J, Thompson CB (1995) Bel-2 and Bcl-2-related proteins in apoptosis regulation. In: Kroemer G, Martinez AC (eds) Apoptosis in Immunology. Springer, Berlin Heidelberg New York, pp 107–121 (Current topics in microbiology and immunology, vol 200)Google Scholar
- Chaly N, Johnstone M, Hand R (1980) Alterations in nuclear structure and function in reovirus-infected cells. Clin Invest Med 2:141–152Google Scholar
- de Jong D, Prins F, Van Krieken HHJM, Mason DY, Van Ommen GB, Kluin PM (1992) Subcellular localization of bel-2 protein. In: Potter M, Melchers F (eds) Mechanisms in B cell neoplasia. Springer, Berlin Heidelberg New York, pp 287–292 (Current topics in microbiology and immunology, vol 176)Google Scholar
- Gauntt CJ (1973) Induction of interferon in L cells by reoviruses. Infection Immun 7:711–717Google Scholar
- Mustoe TA, Ramig RF, Sharpe AH, Fields BN (1978) Genetics of reovirus: identification of the dsRNA segments encoding the polypeptides of the mu and sigma size classes. Virology 89:594–604Google Scholar
- Nibert ML, Schiff LA, Fields BN (1996) Reoviruses and their replication. In: Fields BN, Knipe DM, Howley PM et al (eds) Fields virology, 3rd edn. Raven, New York, pp 1557–1596Google Scholar
- Rodgers SE, Barton ES, Oberhaus SM, Pike B, Gibson CA, Tyler KL, Dermody TS (1997) Reovirusinduced apoptosis of MDCK cells is not linked to viral yield and is blocked by Bel-2. J Virol 71:25402546Google Scholar
- Sarkar G, Pelletier J, Bassel-Duby R, Jayasuriya A, Fields BN, Sonnenberg N (1985) Identification of a new polypeptide coded by reovirus gene SI.I Virol 54:720–725Google Scholar
- Tyler KL, Fields BN (1996) Reoviruses. In: Fields BN, Knipe DM, Howley PM et al (eds) Fields virology, 3rd edn. Raven, New York, pp 1597–1624Google Scholar
- Weiner HL, Ault KA, Fields BN (1980a) Interaction of reovirus with cell surface receptors. I. Murine and human lymphocytes have a receptor for the hemagglutinin of reovirus type 3. J Immunol 124:2143–2148Google Scholar
- Wetzel JD, Wilson GJ, Baer GS, Dunnigan LR, Wright JP, Tang DSH, Dermody TS (1997) Reovirus variants selected during persistent infections of L cells contain mutations in the viral SI and S4 genes and are altered in viral disassembly. J Virol 71:1362–1369Google Scholar