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Gene Transfer by Bacterial Conjugation: Establishment of the Immigrant Plasmid in the Recipient Cell

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Molecular Microbiology

Part of the book series: NATO ASI Series ((ASIH,volume 103))

Abstract

Bacterial plasmids are extrachromosomal DNA elements that replicate autonomously in their host cells. In addition to containing sectors for replication and maintenance stability, plasmids collectively carry diverse cargoes of specialised genes for functions of environmental, medical and commercial importance. Examples are degradative enzymes, antibiotic-resistance and virulence factors, and DNA restriction enzymes.

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References

  • Anthony KG, Sherburne C, Sherburne R, Frost LS (1994) The role of the pilus in recipient cell recognition during bacterial conjugation mediated by F-like plasmids. Mol Microbiol 13: 939–953

    Article  PubMed  CAS  Google Scholar 

  • Balzer D, Pansegrau W, Lanka E (1994) Essential motifs of relaxase (Tral) and TraG proteins involved in conjugative transfer of plasmid RP4. J Bacteriol 176: 4285–4295

    PubMed  CAS  Google Scholar 

  • Bickle TA, Krüger DH (1993) Biology of DNA restriction. Microbiol Rev 57: 434–450

    PubMed  CAS  Google Scholar 

  • Chilley PM, Wilkins BM (1995) Distribution of the ardA family of antirestriction genes on conjugative plasmids. Microbiology 141: 2157–2164

    Article  PubMed  CAS  Google Scholar 

  • Citovsky V, Zupan J, Warnick D, Zambryski, P (1992) Nuclear localization of Agrobacterium VirE2 protein in plant cells. Science 256: 1802–1805

    Article  PubMed  CAS  Google Scholar 

  • Clewell DB (1993) Bacterial sex pheromone-induced transfer. Cell 73: 9–12

    Article  PubMed  CAS  Google Scholar 

  • Dingwall C, Laskey RA (1991) Nuclear targeting sequences-a consensus? Trends Biochem Sci 16: 478–481

    Article  PubMed  CAS  Google Scholar 

  • Dutreix M, Bäckman A, Célérier J, Bagdasarian MM, Sommer S, Bailone A, Devoret R, Bagdasarian M (1988) Identification of the psiB genes of plasmids F and R6–5. Molecular basis for psiB enhanced expression in plasmid R6–5. Nucl Acids Res 16: 10669–10679

    Article  PubMed  CAS  Google Scholar 

  • Erickson MJ, Meyer RJ (1993) The origin of greater-than-unit-length plasmids generated during bacterial conjugation. Mol Microbiol 7: 289–298

    Article  PubMed  CAS  Google Scholar 

  • Feruya N, Komano T (1991) Determination of the nick site at oriT of Inch plasmid R64: global similarity of oriT structures of Inch and IncP plasmids. J Bacteriol 173: 6612–6617

    Google Scholar 

  • Frost LS, Ippen-Ihler K, Skurray RA (1994) Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol Rev 58: 162–210

    PubMed  CAS  Google Scholar 

  • Haase J, Lurz R, Grahn AM, Bamford DH, Lanka E (1995) Bacterial conjugation mediated by plasmid RP4: RSF1010 mobilization, donor-specific phage propagation, and pilus production require the same Tra2 core components of a proposed DNA transport complex. J Bacteriol 177: 4779–4791

    PubMed  CAS  Google Scholar 

  • Heinemann JA, Sprague GF (1989) Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast. Nature 340: 205–209

    Article  PubMed  CAS  Google Scholar 

  • Howard EA, Zupan JR, Citovsky V, Zambryski PC (1992) The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cells. Cell 68: 109–118

    Article  PubMed  CAS  Google Scholar 

  • Jones AL, Barth PT, Wilkins BM (1992) Zygotic induction of plasmid ssb and psiB genes following conjugative transfer of IncIl plasmid ColIb-P9. Mol Microbiol 6: 605–613

    Article  PubMed  CAS  Google Scholar 

  • Lanka E, Wilkins BM (1995) DNA processing reactions in bacterial conjugation. Annu Rev Biochem 64: 141–169

    Article  PubMed  CAS  Google Scholar 

  • Merryweather A, Barth PT, Wilkins BM (1986) Role and specificity of plasmid RP4encoded DNA primase in bacterial conjugation. J Bacteriol 167: 12–17

    PubMed  CAS  Google Scholar 

  • Pansegrau W, Lanka E, Barth PT, Figurski DH, Guiney DG, Haas D, Helinski DR, Schwab H, Stanisich VA, Thomas CM (1994) Complete nucleotide sequence of Birmingham IncPa plasmids: compilation and comparative analysis of sequence data. J Mol Biol 239: 623–663

    Article  PubMed  CAS  Google Scholar 

  • Read TD, Thomas AT, Wilkins BM (1992) Evasion of type I and type II restriction systems by IncII plasmid ColIbP-9 during transfer by bacterial conjugation. Mol Microbiol 6: 1933–1941

    Article  PubMed  CAS  Google Scholar 

  • Rees CED, Wilkins BM (1990) Protein transfer into the recipient cell during bacterial conjugation: studies with F and RP4. Mol Microbiol 4: 1199–1205

    Article  PubMed  CAS  Google Scholar 

  • Simon, JR, Moore PD (1987) Homologous recombination between single-stranded DNA and chromosomal genes in Saccharomyces cerevisiae. Mol Cell Biol 7: 2329–2334.

    PubMed  CAS  Google Scholar 

  • Skurray RA, Reeves P (1973) Physiology of Escherichia coli K-12 during conjugation: altered recipient cell functions associated with lethal zygosis. J Bacteriol 114: 11–17

    PubMed  CAS  Google Scholar 

  • Skurray RA, Reeves P (1974) F factor-mediated immunity to lethal zygosis in Escherichia coli K-12. J Bacteriol 117: 100–106

    PubMed  CAS  Google Scholar 

  • Sundberg C, Meek L, Carroll K, Das A, Ream W (1996) VirEl protein mediates export of the single-stranded DNA binding protein VirE2 from Agrobacterium tumefaciens into plant cells. J Bacteriol 178: 1207–1212

    PubMed  CAS  Google Scholar 

  • Wilkins BM, Chilley PM, Thomas AT, Pocklington MJ (1996) Distribution of restriction enzyme recognition sequences on broad host range plasmid RP4: molecular and evolutionary implications. J Mol Biol 258: 447–456

    Article  PubMed  CAS  Google Scholar 

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© 1998 Springer-Verlag Berlin Heidelberg

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Wilkins, B.M., Bates, S. (1998). Gene Transfer by Bacterial Conjugation: Establishment of the Immigrant Plasmid in the Recipient Cell. In: Busby, S.J.W., Thomas, C.M., Brown, N.L. (eds) Molecular Microbiology. NATO ASI Series, vol 103. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72071-0_3

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  • DOI: https://doi.org/10.1007/978-3-642-72071-0_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72073-4

  • Online ISBN: 978-3-642-72071-0

  • eBook Packages: Springer Book Archive

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