Protoplast-Independent Production of Transgenic Plants
Problems encountered with protoplast-based methods for the generation of transgenic plants have prompted the development of alternative techniques for gene transfer in grasses. These problems relate mainly to plant regeneration from protoplasts and do not reflect specific barriers to the uptake of foreign DNA by isolated protoplasts. Examples of these difficulties are relatively low plating efficiencies and low plant regeneration frequencies from protoplasts, species and genotype dependence often observed in the regeneration process, and albinism and somaclonal variation revealed in protoplast-derived plants (Potrykus 1990). Plant regeneration from protoplasts is thus a delicate process depending upon parameters that are not under experimental control, such as wound response and genotype-dependent competence for regeneration (Vasil 1988; Potrykus 1990). Furthermore, transgenic plants recovered from protoplasts may show serious fertility constraints and undesired integration of multiple and rearranged transgene copies (Spangenberg et al. 1995a).
KeywordsMaize Carbide Mannitol Sorghum Alba
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- Dalton SJ, Bettany AJE, Timms E, Morris P (1998) Transgenic plants of Lolium multiflorum, Lolium perenne, Festuca arundinacea and Agrostis stolonifera by silicon carbide fibre-mediated transformation of cell suspension cultures. Plant Sci (in press)Google Scholar
- Dunder E, Dawson J, Suttie J, Pace G (1995) Maize transformation by microprojectile bombardment of immature embryos. In: Potrykus I, Spangenberg G (eds) Gene transfer to plants. Springer, Berlin Heidelberg New York, pp 127–138Google Scholar
- Hensgens LAM, de Bakker EPHM, van Os-Ruygrok EP, Rueb S, van de Mark F, van der Maas HM, van der Veen S, Kooman-Gersmann M, Hart L, Schilperoort RA (1993) Transient and stable expression of gusA fusions with rice genes in rice, barley and perennial ryegrass. Plant Mol Biol 22: 1101–1127PubMedCrossRefGoogle Scholar
- Klein TM (1995) The biolistic transformation system. In: Potrykus I, Spangenberg G (eds) Gene transfer to plants. Springer, Berlin Heidelberg New York, pp 115–117Google Scholar
- Pérez-Vicente R, Wen XD, Wang ZY, Leduc N, Sautter C, Wehrli E, Potrykus I, Spangenberg G (1993) Culture of vegetative and floral meristems in ryegrasses: potential targets for microballistic transformation. J Plant Physiol 142: 610–617Google Scholar
- Sanford JC, Klein TM, Wolf ED, Allen NJ (1987) Delivery of substances into cells and tissues using a particle bombardment process. J Particulate Sci Technol 6: 559–563Google Scholar
- Spangenberg G, Wang ZY, Wu XL, Nagel J, Iglesias VA, Potrykus I (1995a) Transgenic tall fescue (Festuca arundinacea) and red fescue (F. rubra) plants from microprojectile bombardment of embryogenic suspension cells. J Plant Physiol 145: 693–701Google Scholar
- Spangenberg G, Wang ZY, Potrykus I (1998) Biotechnology in forage and turf grass improvement. In: Frankel R, Grossman M, Linskens HF, Maliga P, Riley R (eds) Monographs on theoretical and applied genetics, this volume. Springer, Berlin Heidelberg New YorkGoogle Scholar
- Wang K, Frame BR, Drayton PR, Thompson JA (1995) Silicon carbide whisker-mediated transformation: regeneration of transgenic maize plants. In: Potrykus I, Spangenberg G (eds) Gene transfer to plants. Springer, Berlin Heidelberg New York, pp 186–192Google Scholar
- Ye XD (1997) Gene transfer to ryegrasses (Lolium spp.): modification of fructan metabolism in transgenic plants. PhD Diss, Swiss Federal Institute of Technology, ZürichGoogle Scholar
- Ye XD, Wang ZY, Wu XL, Potrykus I, Spangenberg G (1997) Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells. Plant Cell Rep 16: 379–384Google Scholar