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Transgenic Plants from Protoplasts

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Biotechnology in Forage and Turf Grass Improvement

Part of the book series: Monographs on Theoretical and Applied Genetics ((GENETICS,volume 23))

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Abstract

Direct gene transfer to protoplasts is one of several methods which have been developed for the generation of transgenic plants and for transient gene expression studies (Potrykus 1990, 1991, 1995; Saul and Potrykus 1990). The method is based on the efficient uptake of plasmid DNA from the surrounding medium into protoplasts which is promoted either by chemical treatment, e.g. with polyethylene glycol (PEG; Paszkowski et al. 1984; Negrutiu et al. 1987), or by the application of electric pulses (electroporation; Fromm et al. 1986), or by a combination of both (Shillito et al. 1986). In those cases where an appropriate protoplast-to-plant regeneration system is available, a large number of transformed clones can be obtained, and often these clones can be regenerated to fully fertile transgenic plants (Davey et al. 1989; Roest and Gilissen 1989; Potrykus 1995).

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Authors and Affiliations

  1. Plant Sciences and Biotechnology, Agriculture Victoria, Department of Natural Resources and Environment and CRC for Molecular Plant Breeding, La Trobe University, 3083, Bundoora, Victoria, Australia

    Prof. Dr. Germán Spangenberg & Dr. Zeng-Yu Wang

  2. Institute of Plant Sciences, Swiss Federal Institute of Technology, 8092, Zürich, Switzerland

    Prof. Dr. Ingo Potrykus

Authors
  1. Prof. Dr. Germán Spangenberg
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  2. Dr. Zeng-Yu Wang
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  3. Prof. Dr. Ingo Potrykus
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© 1998 Springer-Verlag Berlin Heidelberg

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Spangenberg, G., Wang, ZY., Potrykus, I. (1998). Transgenic Plants from Protoplasts. In: Biotechnology in Forage and Turf Grass Improvement. Monographs on Theoretical and Applied Genetics, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72051-2_7

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  • DOI: https://doi.org/10.1007/978-3-642-72051-2_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72053-6

  • Online ISBN: 978-3-642-72051-2

  • eBook Packages: Springer Book Archive

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