Meristem Culture

  • Germán Spangenberg
  • Zeng-Yu Wang
  • Ingo Potrykus
Part of the Monographs on Theoretical and Applied Genetics book series (GENETICS, volume 23)


Shoot meristems, whether apical or axillary, are small domes (50–150µm in diameter) consisting of groups of a few actively dividing cells protected by developing leaves. The main physiological functions of meristems are the synthesis of protoplasm and the production of new cells. The biology and developmental morphology of the shoot apical meristems have been studied in grasses (Evans and Grover 1940; Sharman 1942, 1945, 1947; Peacock 1975; Parsons and Robson 1980; Sweet et al. 1991).


Shoot Apex Shoot Apical Meristem Plant Cell Tissue Organ Cult Turf Grass Meristem Culture 
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  1. Carman JG (1995) Nutrient absorption and the development and genetic stability of cultured meristems. In: Terzi M, Cella R, Falavigna A (eds) Current issues in plant molecular and cellular biology. Kluwer Academic, Dordrecht, pp 393–403CrossRefGoogle Scholar
  2. Dale PJ (1975) Meristem tip culture in Lolium multiflorum. J Exp Bot 26: 731–736CrossRefGoogle Scholar
  3. Dale PJ (1977a) The elimination of ryegrass mosaic virus from Lolium multiflorum by meristem tip culture. Ann Appl Biol 85: 93–96CrossRefGoogle Scholar
  4. Dale PJ (1977b) Meristem tip culture in Lolium, Festuca, Phleum and Dactylis. Plant Sci Lett 9: 333–338CrossRefGoogle Scholar
  5. Dale PJ (1979) The elimination of cocksfoot streak virus, cocksfoot mild mosaic virus and cocksfoot mottle virus from Dactylis glomerata by shoot tip and tiller bud culture. Ann Appl Biol 93: 285–288CrossRefGoogle Scholar
  6. Dale PJ (1980) A method for in vitro storage of Lolium multiflorum Lam. Ann Bot 45: 497–502Google Scholar
  7. Dale PJ, Dalton SJ (1983) Immature inflorescence culture in Lolium, Festuca, Phleum and Dactylis. Z Pflanzenphysiol 111: 39–45Google Scholar
  8. Dale PJ, Webb KJ (1985) Germplasm storage and micropropagation. In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture. Nijhoff/Junk, Dordrecht, pp 79–96CrossRefGoogle Scholar
  9. Dale PJ, Cheyne VA, Dalton SJ (1980) Pathogen elimination and in vitro plant storage in forage grasses and legumes. In: Ingram DS, Helgeson JP (eds) Tissue culture methods for plant pathologists. Blackwell Scientific, Oxford, pp 119–124Google Scholar
  10. Dale PJ, Thomas E, Brettell RIS, Wernicke W (1981) Embryogenesis from cultured immature inflorescences and nodes of Lolium multiflorum. Plant Cell Tissue Organ Cult 1: 47–55CrossRefGoogle Scholar
  11. Dalton SJ, Dale PJ (1981) Induced tillering of Lolium multiflorum in vitro. Plant Cell Tissue Organ Cult 1: 57–64CrossRefGoogle Scholar
  12. Dalton SJ, Dale PJ (1985) The application of in vitro tiller induction in Lolium multiflorum. Euphytica 34: 897–904CrossRefGoogle Scholar
  13. Evans MW, Grover FO (1940) Developmental morphology of the growing point of the shoot and the inflorescence in grasses. J Agric Res 61: 481–520Google Scholar
  14. Humphreys NC, Dalton SJ (1992) A relationship between heading date and in vitro tillering propensity in perennial ryegrass (Lolium per enne L.). Euphytica 58: 253–257CrossRefGoogle Scholar
  15. Hussey G (1978) The application of tissue culture to the vegetative propagation of plants. Sci Progs 65:185–208Google Scholar
  16. Kartha KK (1986) Production and indexing of disease-free plaints. In: Withers LA, Alderson PG (eds) Plant tissue culture and its agricultural applications. Butterworths, London, pp 219–238Google Scholar
  17. King RW, Blundell C, Evans LT (1993) The behaviour of shoot apices of Lolium temulentum in vitro as the basis of an assay system for florigenic extracts. Aust J Plant Physiol 20: 337–348CrossRefGoogle Scholar
  18. McDaniel CN, King RW, Evans LT (1991) Floral determination and in-vitro floral differentiation in isolated shoot apices of Lolium temulentum L. Planta 185: 9–16CrossRefGoogle Scholar
  19. Murashige T (1974) Plant propagation through tissue cultures. Annu Rev Plant Physiol 25: 135–166CrossRefGoogle Scholar
  20. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  21. Pareddy DR, Greyson RI, Walden DB (1987) Fertilization and seed production with pollen from in-vitro-cultured maize tassels. Planta 170: 141–143CrossRefGoogle Scholar
  22. Parsons AJ, Robson MJ (1980) Seasonal changes in the physiology of S24 perennial ryegrass (Lolium perenne L.). 1. Response of leaf extension to temperature during the transition from vegetative to reproductive growth. Ann Bot 46: 435–444Google Scholar
  23. Peacock JM (1975) Temperature and leaf growth in Lolium perenne. III. Factors affecting seasonal differences. J Appl Ecology 12: 685–697CrossRefGoogle Scholar
  24. Pérez-Vicente R, Wen XD, Wang ZY, Leduc N, Sautter C, Wehrli E, Potrykus I, Spangenberg G (1993) Culture of vegetative and floral meristems in ryegrasses: potential targets for microballistic transformation. J Plant Physiol 142: 610–617Google Scholar
  25. Quak F (1977) Meristem culture and virus-free plants. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 598–615Google Scholar
  26. Raman K, Walden DB, Greyson RI (1980) Propagation of Zea mays L. by shoot tip culture: a feasibility study. Ann Bot 45: 183–189Google Scholar
  27. Sautter C (1993) Development of a microtargeting device for particle bombardment of plant meristems. Plant Cell Tissue Organ Cult 33: 251–257CrossRefGoogle Scholar
  28. Sharman BC (1942) Shoot apex in grasses and cereals. Nature 149: 82–83CrossRefGoogle Scholar
  29. Sharman BC (1945) Construction of the shoot apex in cereals and other grasses. Nature 155: 291–292CrossRefGoogle Scholar
  30. Sharman BC (1947) The biology and developmental morphology of the shoot apex in Gramineae. New Phytol 46: 20–34CrossRefGoogle Scholar
  31. Smith RH, Murashige T (1970) In vitro development of the isolated shoot apical meristem of angiosperms. Am J Bot 57: 562–568CrossRefGoogle Scholar
  32. Stapleton AE, Bedinger PA (1992) Immature maize spikelets develop and produce pollen in culture. Plant Cell Rep 11: 248–252CrossRefGoogle Scholar
  33. Sweet N, Wiltshire JJJ, Baker CK (1991) A new descriptive scale for early reproductive development in Lolium perenne L. Grass Forage Sci 46: 201–206CrossRefGoogle Scholar
  34. Trione EJ, Stockwell VO (1989) Development of detached wheat spikelets in culture. Plant Cell Tissue Organ Cult 17: 161–170CrossRefGoogle Scholar
  35. Van Zaayen A, Van Eijk C, Versluijs JMA (1992) Production of high quality ornamental crops through meristem culture. Acta Bot Neerl 41: 425–433Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Germán Spangenberg
    • 1
  • Zeng-Yu Wang
    • 1
  • Ingo Potrykus
    • 2
  1. 1.Plant Sciences and Biotechnology, Agriculture Victoria, Department of Natural Resources and Environment and CRC for Molecular Plant BreedingLa Trobe UniversityBundooraAustralia
  2. 2.Institute of Plant SciencesSwiss Federal Institute of TechnologyZürichSwitzerland

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