Abstract
Since 1971, enzyme-amplified immunoassays have been developed to enhance the detectability of antigen-antibody reactions. The most commonly applied immunoassay is the enzyme-linked immunosorbent assay (ELISA), in which the antigen-antibody complexes are adsorbed to wells in plastic microtitre plates. The most important advantages of ELISA over other serological tests for plant viruses are:
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Very low concentrations of virus (1–10 ng/ml) can be detected.
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Only small amounts of antibodies are required.
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The test can be applied to both crude virus preparations as well as purified virus suspensions.
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The test is suitable for large-scale testing of samples.
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The test can be standardised using kits.
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Quantitative measurements are possible.
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© 1998 Springer-Verlag Berlin Heidelberg
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Dijkstra, J., de Jager, C.P. (1998). Enzyme-Linked Immunosorbent Assay. In: Practical Plant Virology. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72030-7_56
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DOI: https://doi.org/10.1007/978-3-642-72030-7_56
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48981-5
Online ISBN: 978-3-642-72030-7
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