Enzyme-Linked Immunosorbent Assay

  • Jeanne Dijkstra
  • Cees P. de Jager
Part of the Springer Lab Manual book series (SLM)

Abstract

Since 1971, enzyme-amplified immunoassays have been developed to enhance the detectability of antigen-antibody reactions. The most commonly applied immunoassay is the enzyme-linked immunosorbent assay (ELISA), in which the antigen-antibody complexes are adsorbed to wells in plastic microtitre plates. The most important advantages of ELISA over other serological tests for plant viruses are:
  • Very low concentrations of virus (1–10 ng/ml) can be detected.

  • Only small amounts of antibodies are required.

  • The test can be applied to both crude virus preparations as well as purified virus suspensions.

  • The test is suitable for large-scale testing of samples.

  • The test can be standardised using kits.

  • Quantitative measurements are possible.

Keywords

Starch Iodine Glycine Penicillin Polystyrene 

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References

  1. Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34: 475–483PubMedCrossRefGoogle Scholar
  2. Clark MF, Bar-Joseph M (1984) Enzyme immunosorbent assays in plant virology. Methods Virol 7: 51–85Google Scholar
  3. Flegg CL, Clark MF (1979) The detection of apple chlorotic leafspot virus by a modified procedure of enzyme-linked immunosorbent assay ( ELISA ). Ann Appl Biol 91: 61–65CrossRefGoogle Scholar
  4. Hobbs HA, Reddy DVR, Rajeshwari R, Reddy AS (1987) Use of direct antigen coating and protein A coating ELISA procedures for detection of three peanut viruses. Plant Disease 71: 747–749CrossRefGoogle Scholar
  5. Jayasinghe U, Salazar LF (eds) (1993) Manual de tecnicas en virologia de plantas. Unidad Tecnica de Capacitacion (TTU). CIP, Lima, PeruGoogle Scholar
  6. Koenig R, Paul HL (1982) Variants of ELISA in plant virus diagnosis. J Virol Methods 5: 113–125PubMedCrossRefGoogle Scholar
  7. Stanley CJ, Johannsson A, Self CH (1985) Enzyme amplification can enhance both the speed and the sensitivity of immunoassays. J Immunol Methods 83: 89–95PubMedCrossRefGoogle Scholar
  8. Sudarshana MR, Reddy DVR (1989) Penicillinase-based enzyme-linked immuno- sorbent assay for the detection of plant viruses. J Virol Methods 26: 45–52PubMedCrossRefGoogle Scholar
  9. Torrance L (1987) Use of enzyme amplification in an ELISA to increase sensitivity of detection of barley yellow dwarf virus in oats and in individual vector aphids. J Virol Methods 15: 131–138PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jeanne Dijkstra
    • 1
  • Cees P. de Jager
    • 1
  1. 1.Department of VirologyWageningen Agricultural UniversityWageningenThe Netherlands

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