Enzyme-Linked Immunosorbent Assay

  • Jeanne Dijkstra
  • Cees P. de Jager
Part of the Springer Lab Manual book series (SLM)


Since 1971, enzyme-amplified immunoassays have been developed to enhance the detectability of antigen-antibody reactions. The most commonly applied immunoassay is the enzyme-linked immunosorbent assay (ELISA), in which the antigen-antibody complexes are adsorbed to wells in plastic microtitre plates. The most important advantages of ELISA over other serological tests for plant viruses are:
  • Very low concentrations of virus (1–10 ng/ml) can be detected.

  • Only small amounts of antibodies are required.

  • The test can be applied to both crude virus preparations as well as purified virus suspensions.

  • The test is suitable for large-scale testing of samples.

  • The test can be standardised using kits.

  • Quantitative measurements are possible.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jeanne Dijkstra
    • 1
  • Cees P. de Jager
    • 1
  1. 1.Department of VirologyWageningen Agricultural UniversityWageningenThe Netherlands

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